NMR能够在溶液状态下提供高分辨率的蛋白质三维结构,这是X射线晶体学等其它技术难以实现的优点。 氢-氢距离:通过NOESY(Nuclear Overhauser Effect Spectroscopy)等二维NMR技术,测量氢核之间的空间距离。 结构基团:分析蛋白质的二级结构(如α螺旋、β折叠)和三级结构。 核酸结构研究 NMR可用于研究DNA和RNA的结构及其与其他...
K. M. Sanders and B. K. Hunter Modern NMR Spectroscopy (OUP)。 这里也有一些文本采用了在不同的层面上更基于理论的方法:P. J. Hore Nuclear Magnetic Resonance (OUP)。 为了更深入的了解,可参考最近出版的 M. H. Levitt Spin Dynamics (Wiley)。 Acknowledgements Chapter 2到5是专门为本课程准备的。C...
内容提示: The Nuclear Magnetic Resonance Society of Japan EditorExperimental Approaches of NMR Spectroscopy IIApplication to Life Science and Materials ScienceSecondEdition 文档格式:PDF | 页数:421 | 浏览次数:1 | 上传日期:2025-03-26 00:03:57 | 文档星级: The Nuclear Magnetic Resonance Society of...
NMR spectroscopyPSYCHEpure shift NMRBroadband homodecoupling techniques in NMR, also known as "pure shift" methods, aim to enhance spectral resolution by suppressing the effects of homonuclear coupling interactions to turn multiplet signals into singlets. Such techniques typically work by selecting a ...
COSY谱(Correlation Spectroscopy)是最简单且用途最广的二维实验。COSY是通过J耦合建立的自旋核与自旋核之间的联系,也叫化学位移相关谱。同核COSY谱图中的交叉峰表明了邻近同核原子之间的耦合。另外,当一维谱图中谱峰重叠或变宽导致谱图难以解析时,COSY可以辅助谱峰信号的归属。COSY实验不仅可以用于观察1H-1H等同核原...
拉普拉斯核磁共振波谱(Laplace NMR spectroscopy)技术通过检测与分子动力学和自旋相互作用相关的扩散或弛豫等信息,为研究分子的化学结构和物理环境提供了一种强大的工具,因此被广泛应用于化学、材料科学、生物医学、食品科学等多个领域。然而,拉普拉斯NMR波谱的有效性在很大程度上依赖于数据后处理算法从实验采集的指数衰减信号...
Infrared (IR) and Raman Spectroscopy (RS) depend on vibration or rotation of bonded atoms. Nuclear Magnetic and Electric Quadrupole Resonance (NMR, NQR) depend on transitions between quantized energy levels for interactions of the magnetic dipole and electric quadrupole of an atomic nucleus with ...
Laser absorption Spectroscopy Cavity ring down Spectroscopy Luminescence Chemiluminescence Phosphorescence Magnetic Resonance Electron paramagnetic resonance Electron spin resonance Magnetic Resonance Imaging Nuclear Magnetic Resonance (NMR) NMR Permanent magnet NMR Superconducting magnet NMR Tubes NMR Cryoprobes NMR ...
NMR spectroscopy is based on the measurement of the energy differences between different spin states of these nuclei, induced by the application of radiofrequency (RF) pulses. By varying the frequency and timing of these pulses, it is possible to manipulate the nuclear spin states and study their...