For shifting conformation equilibria by anesthetics, V236 of α4 gave a good example. In the second scenario, V236 had two populations (75% vs. 25%) with distinct resonance frequencies in the absence of halothan
In principle, δ1H should reflect the acid strength of Si–OH–Al bridging hydroxyl group, shifting to low field as the polarization of the O–H bond, and thus the acid strength, increases. However, δ1H can be strongly affected by hydrogen bonds to framework oxygen atoms, thus giving ...
We detected in the representative 19F-NMR spectra obtained at different reaction time points (i.e., 6 min, 200 min, and 1000 min, Fig. 4b) a notable consumption of free fluoride (peak at −120 ppm). This obser- vation implies a growth mechanism that differs from particle- 4 NATURE ...
We show that allosteric signal transmission initiated by agonist binding leads to the population of different inactive and pre-active receptor states that are in equilibrium with each other, gradually shifting the receptor towards a more active state as higher efficacy ligands are used. Coupling of ...
In the D150N mutant, the peak positions were slightly different from those in the wild type (Fig. 4c). If the observed differences in the peak positions reflect the shift in the conformational equilibrium in the D150N mutant, then the corresponding 1H-13C MQ chemical shift differences, that...
The use of a spatially shifting excitation to reduce the inter-scan delay was already mentioned in Section 6.2 for rapid-pulsing pure shift methods [118]. The same groups have used the combination of a selective excitation pulse and a gradient for the real-time 1H 1D NMR monitoring of a dy...
B, Ile residues that shift upon the interaction with p23, plotted on one monomer of the homology model of the Hsp90β (shifting residues, red; non-shifting residues, orange). The monomers of Hsp90β are depicted in grey and the location of p23 based on the X-ray structure from yeast...
31P NMR spectra of samples oriented on glass plates with the bilayer normal (perpendicular to the plane of the plates) aligned parallel to the magnetic field typically show a small ‘extra’ peak corresponding to signal arising from the 90° orientation (presumably due to a powder or unoriented...
Note that a general feature of reaction monitoring with 2D NMR, be it ultrafast or not, is that the linear relation between NMR peak volumes and concentration that is used for quantitation becomes peak-dependent, with a response factor that is a complicated and unknown function of the NMR para...
(DSS) was added into each sample, and its concentration was used as a reference to allow relative quantification comparisons between samples to be made. We created an automatic peak picking program to generate peak lists for each sample, where each resonance peak was converted into an X, Y ...