本论文旨在研究NLRP3在TNFα促LDL跨内皮穿胞中的作用,同时制备靶向炎症激活内皮细胞的脂质体siRNA传递系统并考察其效果。 方法(1)在转染NLRP3 siRNA的人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)中加入TNFα刺激,用蛋白免疫印记法(Western Blot)检测NLRP3、IL-1β、caspase-1等炎症小体相关...
接下来,为了研究 VRAC 在 NLRP3 炎症小体和先天性免疫功能中的作用,作者将 Lrrc8afl/fl 小鼠与 LysMCre 小鼠杂交,产生了在髓系细胞中有条件消减 Lrrc8a 的小鼠。与 Lrrc8afl/fl 小鼠相比,从 Lrrc8afl/flLysMCre 小鼠(以下简称 Lrrc8amKO)分离的 BMDMs 中 Lrrc8a 的 mRNA 和蛋白表达明显减少。膜片钳实...
电针百会、大椎能够通过NLRP3/Caspase-1通路改善Aβ25-35诱导的AD模型小鼠的学习记忆能力,降低海马组织IL-1β和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)等炎症因子水平[40]。有研究发现,电针百会能显著改善认知功能障碍小鼠的学习记忆功能,降低脑组织中小胶质细胞活化水平;而使用了NLRP3激活剂后,逆转电针...
The levels of tumor necrosis factor-α (TNF- α), interleukin (IL) -1β and IL-6 in the serum and kidney tissues were detected by ELISA and RT-qPCR. Western blot was used to detect the protein expression levels of NLR family pyrin domain containing protein 3 (NL...
新药|香雷糖足膏获批糖尿病足部伤口溃疡 | 新药|1.1类创新药香雷糖足膏获批上市,用于糖尿病足部伤口溃疡。香雷糖足膏由两种植物活性药物成分组成,通过抑制NLRP3介导的炎症小体信息路径和抑制炎症期的下游炎性细胞因子如白细胞介素(IL)-6、肿瘤坏死因子(TNF)-α、和IL-1β的产生,降低M1巨噬细胞的活性,并通过活化...
In contrast, in LC AMs, we observed a different profile of IL-1β secretion, characterized mainly by the impairment of IL-1β production in NLRP3 stimulated cells. AMs also exhibited an impaired TLR4/LPS pathway as shown by the reduced induction of IL-6 and TNF-α. Our results support ...
虽然WA具有诱导肝脏NRF2和自噬信号通路的作用,但在本研究的实验条件下,其肝保护作用对这两个信号通路的依赖性较小。这些结果表明,WA靶向巨噬细胞治疗GalN/ lps诱导FH,这进一步支持了靶向肝巨噬细胞治疗肝病的可能性,并支持了WA作为一种肝保护剂对抗临...
These data indicate that the sAβ1-42 may play a dual role during inflammatory process, wherein, it may be involved in protecting the cells from inflammatory damage due to TNF-α. This ability of sAβ1-42 might be playing some role in protecting the brain cells during the process of ...
研究表明,NLRP3 炎症小体缺失可诱导 小胶质细胞向 M2 表型转变从而减少 Aβ 在 AD 模型 中的沉积 。[32] 已有研究表明,急性肝损伤中绿原酸 可抑制 NLRP3 炎症小体激活 ,降低 Pro-caspase-1、 caspase-1、Pro-IL-1β 和 IL-1β 蛋白表达。此外,绿原 酸还可降低血清和肝脏 TNF-α、IL-6 和 IL-1β...
After the modeling was complete, rat synovial tissue and serum were analyzed using Hematoxylin and Eosin staining, Western Blot, qRT-PCR, and ELISA to detect the expression of proteins and mRNA related to Piezol, NF-κB, NLRP3, ASC, Caspase-1, IL-1β, IL-6, and ...