CD3+CD56+NKT细胞分离试剂盒用于从人PBMC和其他单细胞悬浮液中顺序分离CD3+CD56+天然杀伤(NK)T细胞。 NK T细胞代表具有NK细胞特性的T细胞亚群。 NKT细胞可以通过与抗原或细胞因子如IL-12接触来刺激,以释放大量细胞因子并发挥细胞毒作用。 NKT细胞是先天免疫系统的关键部分,参与自身免疫疾病的发展,肿瘤免疫学以及对病毒...
CD3+ CD56+ NKT Cell Isolation Kit, human/人CD3+ CD56+NK T细胞分选试剂盒产品类型:T细胞分选品牌:Miltenyi货号:130-093-064 规格:用于 2×10^9 细胞分选 价格:14161加入购物车 产品介绍 技术参数 相关产品 产品介绍CD3 + CD56 + NKT细胞分离试剂盒用于从人PBMC和其他单细胞悬浮液中顺序分离CD3 + CD56 +...
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NK细胞又称自然杀伤细胞(Natural Killer Cell),是机体的一类固有免疫细胞,主要分布于外周血、肝脏和脾脏。作为人体抵抗癌细胞和病毒感染的第一道防线,具有对肿瘤的直接溶解活性,可非特异性直接杀伤肿瘤细胞,这种天然杀伤活性既不需要抗原致敏,也不需要抗体参与。除了具有强大的杀伤功能外,还具有很强的免疫调节功能,与机...
NKT-cell isolation, transduction, and expansion NKT cell lines were expanded from PBMCs of healthy volunteers and transduced with retroviral vectors carrying CAR.GD2 as previously described14,16 with modifications (supplemental Methods). Monocyte isolation and generation of M2 macrophages Monocytes were ...
ARTICLE Received 16 Mar 2015 | Accepted 13 Aug 2015 | Published 18 Sep 2015 DOI: 10.1038/ncomms9371 OPEN Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling Young Jun Kang1, Bo-Ram Bang1, Kyung Ho Han2, Lixin Hong1, ...
The process of cell isolation and expansion is essential due to the small number of NKT cells in peripheral blood circulation. Different approaches can be used to expand NKT cells. NKT cell collection from different sources may be efficient; for example, NKT cells from PBMCs present more α-Ga...
For NK cell isolation, splenocytes were prepared from young adult mice (6–8 week old), incubated with biotinylated monoclonal antibodies for CD4, CD8, CD19, MHC Class II, and Ly-6G and anti-biotin microbeads (Miltenyl) to deplete unwanted cells by negative selection. The NK cells ...
Cell harvesting and isolation All experiments were approved by the institutional animal care committee at the University Hospital Regensburg. Different lymphocytes subsets were purified from splenic or hepatic mononuclear cells isolated from Balb/c mice or C57Bl/6NCrl mice (Charles River Laboratories, Wil...
During the isolation of cells from the ocular surface, epithe- lial cells are mixed with T cells. To address the possibility that epithelial cell contaminants in the NK/NKT + population could be producing IL-13, we performed a magnetic depletion of all bone marrow-derived cells from the ...