全基因合成双特异性靶向人claudin18.2和nkg2dl的嵌合抗原受体分子的核苷酸序列(南京一道生物合成)见seq id no.19或seq id no.20,通过分子克隆的方式连接到慢病毒载体lentiguide-puro(addgene,美国),构建单一编码框的全长car序列表达框,利用ef1alpha启动子或efs启动子进行表达。 [0145] 具体操作步骤如下: [0146] ...
DNA methylation is closely related to the expression of NKG2D-L, which is a critical epigenetic mechanism of gene regulation, and contributes to the downregulation and absence of NKG2D-L expression in AML patients [90,91]. Treatment with DNA methyltransferase (DNMT) inhibitors such as azacitidine ...
Here, we show that a sixth gene, ULBP6/RAET1L, is a polymorphic locus that expresses a functional transcript. ULBP6 had a more restricted expression profile in cell lines and primary human tissues than other NKG2D ligands, but expression was detected in several human papillomavirus-positive ...
Chronic myeloid leukemia (CML) is a myeloproliferative disorder involving the hematopoietic stem cell compartment that is hallmarked by a typical reciprocal translocation between chromosomes 9 and 22. The formed bcr-abl fusion gene is translated into the BCR-ABL oncoprotein which displays constitutive tyr...
Lopez-Soto A, Folgueras AR, Seto E, Gonzalez S. HDAC3 represses the expression of NKG2D ligands ULBPs in epithelial tumour cells: potential implications for the immunosurveillance of cancer. Oncogene (2009) 28:2370-82. doi:10.1038/onc.2009.117...