目的:探讨体内条件下舒尼替尼对耐药鼻咽癌CNE2/DDP细胞NKG2DLs(natural killer group 2 member D ligands)表达的诱导作用,及其对NK细胞抗肿瘤活性的影响。方法:建立ABCG2highCNE2/DDP和ABCG2lowCNE2/DDP细胞裸鼠皮下移植瘤模型,分如下8组:A、E组分别接种ABCG2high、ABCG2lowCNE2/DDP细胞,B、F组分别接种舒...
舒尼替尼体内诱导耐药鼻咽癌细胞表达NKG2DLs增强NK细胞的抑瘤作用
目的:初步探讨舒尼替尼诱导高、低表达ABCG2(ATP-binding cassette superfamily G member 2)分子的耐药鼻咽癌CNE2/DDP细胞(简称ABCG2highCNE2/DDP细胞、ABCG2lowCNE2/DDP细胞)中NKG2D配体(natural killer group 2 member D ligands,NKG2DLs)表达的分子机制.方法:Caspase-8活化试剂盒和线粒体膜电位法分别检测NK细...
#射雕英雄传# 射雕的人物,优缺点都很明显,没有完人,也没有纯粹的恶人。就算立场敌对也可能建立感情,建立了感情也可能为了利益而抛却割舍,抛却割舍了又可能因为新的体悟而再续前缘。经得起立场和利益考验的感情,就走到了最后,比如郭靖黄蓉这对乱世湍流中的小情侣,虽然分分合合,还是牵马纵雕,并肩而行。金庸的长篇...
Serum levels of sNKG2DLs were quantified through the use of ELISA and correlated with clinical data. The analyzed sNKG2DLs were low to absent in HVs and significantly higher in BC patients. For some of the ligands analyzed, higher sNKG2DLs serum levels were associated with the...
目的:探讨舒尼替尼促进肝癌细胞自然杀伤细胞2族成员D配体(natural killer group 2 member D ligands,NKG2DLs)表达及提高NK细胞杀伤肿瘤细胞的作用.方法:常规体外培养HepG2细胞,免疫磁珠法从健康志愿者外周静脉血中分选 NK细胞,流式细胞技术检测NK细胞纯度及1μmol/L舒尼替尼孵育24h前后肝癌细胞NKG2DLs表达率;LDH...
Since MMP inhibitors, MMPi III and MMP2 inhibitor I, restored the reduced expression of NKG2DLs after treatment of TGF-β, it was thought that TGF-β induced the expression of MMP2 which facilitated the shedding of the NKG2DLs in cancer cells. However, the expression of PD-L1, L2 were ...
CD123 and NKG2D ligands (NKG2DLs) are commonly used targets for CAR-T therapy of AML, and M-MDSCs and M2 cells express both antigens. We developed dual-targeted CAR-T (123NL CAR-T) cells targeting CD123 and NKG2DL by various structural optimization screens. Our study reveals that 123...
In this study, we showed that TGF-β up-regulated the expression of NKG2DLs in both PC3 and HepG2 cells. The up-regulation of NKG2DLs was characterized by increasing the expression of UL16-binding proteins (ULBPs) 1 and 2. TGF-β treatment also increased the expression of transcription ...
NKG2DLsImmune escapeIndoleamine-2,3-dioxygenase 1 (IDO1) is an important enzyme for altering the tumour microenvironment and assisting tumour cells to escape the immune system.In this study, a significant reduction in NK cell cytotoxicity that was associated with a high expression of IDO1 in a ...