Freshly isolated NK cells were cultured in NK MACS® Medium (Miltenyi Biotec) supplemented with 5% heat-inactivated (h.i.) human plasma (DRK-Blutspendedienst), 1% NK-MACS® Supplements (Miltenyi Biotec) and
h. 采集乳腺癌组织样本,立即转移至MACS Tissue Storage Solution,可在4°C保存48h。 4.操作步骤 4.1 解离乳腺癌组织为单细胞悬液并富集肿瘤细胞 时间:4h 4.1.1 肿瘤样本处理(按照Miltenyi“Tumor Dissoication Kit, human”方案)1.在生物安全柜中操作,尽可能保持无菌。2.取培养皿加入足量RPMI,称重,移入组织,...
Miltenyi常规培养基NK MACS Medium原装进口 价格 价格面议 发货地 北京 商品类型 化工能源 、 抗体试剂 、 分子生物试剂 商品图片 商品参数 品牌: Miltenyi 有效成分含量: 95% 是否进口: 是 用途范围: 仅供工业或者科研使用 产品类型: 常规培养基 是否现货: 是 发货地: 六大现货仓就近发货 运费: ...
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58.用MACS缓冲液重悬,计数细胞。4.4.2 从PBMC阴性选择分离NK细胞(按照Miltenyi“NK Cell Isolation Kit, human”方案)59.加入MACS缓冲液至50mL。60.室温280×g离心8min(ac=9,dc=9)。61.按每107细胞40μL重悬于预冷的MACS缓冲液。62.4°C孵育5min。63.每107细胞加10μL抗体鸡尾酒。
Digested tumors were then filtered through a 70 μm-pore size MACS SmartStrainer (Miltenyi Biotec) and washed with RPMI 1640 medium. For A20 tumors, this step was followed by purification with mouse CD19 Microbeads (Miltenyi Biotec). Spleens were mechanically dissociated with GentleMACS dissociator...
PBMCs were isolated by a density gradient with Biocoll (Biozol, Germany) followed by indirect magnetic immunoselection (Miltenyi Biotec, Germany) and activation in X-Vivo10 medium (Lonza, Switzerland) supplemented with 5% human serum (Life Technologies, USA), 1000 IU/ml IL-2 (Promokine, ...
Freshly isolated NK cells were grown in 24-well plates in NK MACS Medium (Miltenyi Biotech, Bergisch Gladbach, Germany) supplemented with 100 U/mL IL-2 (Hoffmann La-Roche, Basel, Switzerland) and irradiated K562-mbIL21 feeder cells at an NK: feeder ratio of 4:5. The cells were cultured...
Following density separation, CD14+ monocytes, CD4+ T and CD8+ T cells were isolated by MACS System (MiltenyiBiotec, Bergisch Gladbach, Germany) according to the manufacturer's instructions. DCs were generated as described previously from CD14+ cells.32 Briefly, selected CD14+ cells were cultured...