We have applied resonance Raman spectroscopy to investigate the properties of the dinuclear center of oxidized, reduced, and NO-bound nitric-oxide reductase from . The spectra of the oxidized enzyme show two di
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In denitrifying microorganisms, electrons derived from the oxidation of organic or inorganic substrates are transferred to the different N-oxide reductases through an electron transport chain45.Ca. Nitricoxidivorans perseverans andCa. Nitricoxidireducens bremensis possessed the genes for a NADH–quinone...
3.RESPIRATORY NITRIC OXIDE REDUCTASES ARE MEMBERS OF THE HEME–COPPER OXIDASE SUPERFAMILY Thecatalytic domainof NorZ and the NorB subunit of respiratory NORs are homologous to each other and, in turn, are homologous to thecatalytic subunitof the heme– copperoxidasefamily. The structural similarity ...
(kuste3055) was down-regulated 36-fold. In line with earlier reports, transcripts of genes encoding the catalytic subunit of the cytochromecd1nitrite reductase (nirS, kuste4136) and its accessory proteins (kuste4137–kuste4140) were detected in low amounts even under normal nitrite-dependent ...
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Over the last decade, it has become clear that the functions of vascular endothelium are influenced by atherosclerosis. Conversely, atherogenesis is modulated by mediators released by the endothelial cells as well. Nitric oxide (NO) is one of those media
总一氧化氮检测试剂盒(Total Nitric Oxide Assay Kit, 即Nitrate/Nitrite Assay Kit)采用了硝酸盐还原酶(Nitrate reductase)还原硝酸盐(nitrate)为亚硝酸盐(nitrite),然后通过经典的Griess reagent检测亚硝酸盐,从而测定出总一氧化氮。一氧化氮本身极不稳定,在细胞内很快代谢为硝酸盐和亚硝酸盐,通过上述方法检测出所有...
Nitric oxide (NO) is a signalling molecule involved in several physiological processes, in both prokaryotes and eukaryotes, and nitrite is being recognised
The structure reveals that the heme irons in each subunit exhibit a rare His/Cys ligation, which, as we show by substitution, causes the observed unusual spectral properties. Unlike its individual subunits, the KsNaxLS complex binds nitric oxide (NO) only at the distal heme side, forming 6...