The invention provides respiratory nitrate reductase alpha subunit polypeptides and polynucleotides encoding respiratory nitrate reductase alpha subunit polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing respiratory nitrate reductase alpha ...
heterotrophic denitrifying bacteria that are unable to use sulfur compounds as electron donors28,29,30, these MAGs also encode various enzymes for sulfur oxidation (Fig.3cand Supplementary Fig.5), the most prevalent being sulfide:quinone oxidoreductase (SQR), a key enzyme that can catalyze sulfide ...
Removal of the high-potential [4Fe–4S] center of the beta-subunit from Escherichia coli nitrate reductase. Physiological, biochemical, and EPR characterization of site-directed mutated enzymes Biochemistry, 32 (1993), pp. 5099-5108 CrossrefView in ScopusGoogle Scholar [30] J. Buc, C.L. Sant...
NiR, ferredoxin-NADP reductase (FNR) and ferredoxin (Fig. S8), suggesting the potent role of light in nitrate response. To understand the global function of PPI networks for differential nitrate responses in light and dark, the DEG-associated interactors were subjected to GO enrichment analysis ...
Thermomonas delftensis” genome assembly mainly harbored respiratory nitrate reductases (alpha, beta, delta, and gamma chains), and some denitrification gene homologues (nir, nor). 4. Discussion 4.1. Effect of different operating conditions on anoxic phosphorus uptake activity The anoxic phosphorus upt...
The periplasmic reduction of the electron acceptors nitrate (Em +420 mV) and trimethylamine-N-oxide (TMAO; Em +130 mV) by Nap and Tor reductases is widespread in Gram-negative bacteria and is usually considered to be driven by non-energy cons
Nitrate reductases can reduce NO 3 À to NO 2 À and be divided into NAR and NAP complexes. Denitrifying bacteria are reported to contain one or both of the nitrate reductases (Carter et al., 1995; Roussel et al., 2005). NAR complex is membrane-bound with the NarGcatalytic protein...
the NapB subunit (15 kDa) uses two c-type hemes to mediate electrons from NapC to NapA. The strength of the interaction between NapA and NapB is organism-dependent: nitrate reductases from E. coli or S. gelidimarina purify as a monomer (NapA), while nitrate reductases from P. panto...
The invention provides respiratory nitrate reductase alpha subunit polypeptides and polynucleotides encoding respiratory nitrate reductase alpha subunit polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing respiratory nitrate reductase alpha ...
A soluble αβ complex of nitrate reductase can be obtained from a strain of Escherichia coli that lacks the narI gene and expresses only the α and β subunits. The β subunit contains four Fe-S centres and the α subunit contains the molybdenum cofactor, which is the site at which ...