Western blot analysis of extracts from mouse primary bone marrow-derived macrophage (BMDM) cells, untreated (-), or treated (+) with Lipopolysaccharides (LPS) #14011 (50 ng/ml, 4 hr) and Nigericin (sodium salt)
Pune) in RPMI1640 containing 15 mM HEPES, 50 mg/L Gentamicin sulfate 10 mg/L hypoxanthine, 2 g/L sodium bicarbonate (Sigma-Aldrich) and 2.5 g/L AlbuMAX