Moreover, the concentration of purified eGFP by PDA/Fe3+/Ni2+ was reported 138.83 mu g/ml by the fluorescent signals, which is almost equal to or more than the protein purified by commercial Ni-NTA column (108.28 mu g/ ml). The stability of PDA/Fe3+/Ni2+ has also been evaluated ...
Chelating HP column 用Ni2+-螯合HP柱纯化E.coli表达的(His)6-tagged重组蛋白包含体 A chromatographic method for the purification of (His)6-tagged recombinant proteins expressed as inclusion bodies in Escherichia coli is described. The inclusion bodies are solubilized in 8 M urea and bound to a Ni...
L00250-2-High-Affinity Ni-NTA Resin
AuNP CPD-dried hydrogels and the coverslips after particle transfer to hydrogels – were sputter-coated with carbon (Low Vacuum Coater EM ACE200, Leica) and inspected in an Ultra 55 F E-SEM featuring a Gemini column (Carl Zeiss) using an in-lens detector at accelerating voltages of 3...
Purification and on-column refolding of His-tagged PhaZ depolymerase from Pseudomonas putida KT2442 on Ni2+NTA agarose gel: preparation of a novel immobilized biocatalystPichia pastorisFed-batch cultivationFormaldehyde dehydrogenase promoterRhizopus oryzae lipase...
The purification of the iNOS/CaM complex from 4L BL21 (DE3) culture had been performed on 2', 5' 鈥揂DP Sepharose, NiNTA, and S200 gel filtration column. The purified active iNOS had been collected from only NiNTA column with 89% total iNOS yield and 200-fold purification. However, ...
Removal of nickel(II) ions from aqueous solution by biosorption in a fixed bed column: Experimental and theoretical breakthrough curves. Biochem. Eng. J. 2006, 30, 184–191. [CrossRef] 3. Boujelben, N.; Bouzid, J.; Elouear, Z. Adsorption of nickel and copper onto natural iron oxide-...
The concentration of the main dopant in molar percentage is listed in the Main Dopant column. Main Dopant Co (6%, 45%, 75%) Co (20%, 55%) Fe (10%, 20%, 30%) Al, Fe, Cr, Mn (25%) Al (10%–25%) Al (20%) None Preparation Method EP CP CP EP CP from nitrates CP from...
The separation of H2, CH4, and CO was achieved with a Molecular Sieve 5A column (10 ft ˆ 1/8 in), whereas CO2, H2O, C2H4, C2H6, C2H5OH, CH3CHO and CH3COCH3 were separated in a Poraplot Q column (10 ft ˆ 1/8 in) in a series bypass configuration. The equations used to ...