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Moreover, the concentration of purified eGFP by PDA/Fe3+/Ni2+ was reported 138.83 mu g/ml by the fluorescent signals, which is almost equal to or more than the protein purified by commercial Ni-NTA column (108.28 mu g/ ml). The stability of PDA/Fe3+/Ni2+ has also been evaluated ...
Product Name Index NI-424 (IC) Applications
Chelating HP column 用Ni2+-螯合HP柱纯化E.coli表达的(His)6-tagged重组蛋白包含体 A chromatographic method for the purification of (His)6-tagged recombinant proteins expressed as inclusion bodies in Escherichia coli is described. The inclusion bodies are solubilized in 8 M urea and bound to a Ni...
Purification and on-column refolding of His-tagged PhaZ depolymerase from Pseudomonas putida KT2442 on Ni2+NTA agarose gel: preparation of a novel immobilized biocatalystPichia pastorisFed-batch cultivationFormaldehyde dehydrogenase promoterRhizopus oryzae lipase...
The purification of the iNOS/CaM complex from 4L BL21 (DE3) culture had been performed on 2', 5' 鈥揂DP Sepharose, NiNTA, and S200 gel filtration column. The purified active iNOS had been collected from only NiNTA column with 89% total iNOS yield and 200-fold purification. However, ...
The concentration of the main dopant in molar percentage is listed in the Main Dopant column. Main Dopant Co (6%, 45%, 75%) Co (20%, 55%) Fe (10%, 20%, 30%) Al, Fe, Cr, Mn (25%) Al (10%–25%) Al (20%) None Preparation Method EP CP CP EP CP from nitrates CP from...