产品编号: CS210432 查看说明书 产品名称: Ni-NTA Superflow, 50% Slurry .0 订购此产品 供应商: Millipore 规格: ML 目录价: 63.00 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址: 点击查看详细 打印此页 关闭此页 上一个:Ni-NTA Agarose, 50% Slurry[CS210431/...
High Affinity Ni-NTA Resin (Cat. No. L00250, 25 ml as 50 ml of 50% slurry) is an agarose resin (4% cross-linked) covalently coupled to a tridentate chelating agent (NTA) that binds Ni2+ ions by four coordination sites for high-affinity purification of His-tagged recombinant proteins ...
Adust pH to 8.0 using NaOH Washing Buffer (1 liter) 50 mM NaH2PO4 6.90g NaH2PO4.H2O(MW 137.99 ) 300 mM NaCl(up to 2M)17.54g NaCl (MW 58.44 )or 60ml 5 M 20 mM Imidazole(up to 100mM)1.36g Imidazole (MW 68.08 ) 10% Glycerol 100ml 100% stock 10 mM BME 0.69ml stock (14.4M )...
Ni-NTA Agarose (100 ml) R901-10 System Components The Ni-NTA Purification System components are listed below and include enough resin, reagents, and columns for six purifications. Component Composition Quantity Ni-NTA Agarose 50% slurry in 30% ethanol 10 ml ...
…… 6 I. DESCRIPTION High Affinity Ni-NTA Resin (Cat. No. L00250, 25 ml as 50 ml of 50% slurry) is an agarose resin (4% cross-linked) covalently coupled to a tridentate chelating agent (NTA) that binds Ni2+ ions by four coordination sites for high-affinity purification of His-...
Stationary PhaseNi-NTA TypeAgarose Quantity100 mL FormSuspension Column TypeAffinity Column Unit SizeEach 內容物與存放 The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will ...
IDA、NTA和TED分别拥有3、4、5个配位位点与镍离子结合,这样镍离子空余的能与组氨酸标签结合的位点为3、2、1个,所以TED与His标签蛋白结合能力较弱,但其特异性强,在高浓度螯合剂、还原剂和碱性环境下具有良好的耐受性,而IDA则相反,可结合较多的His标签蛋白,但特异性较弱。NTA与His标签蛋白的作用介于两者之间。
Form: Suspension in aqueous solution with 30% isopropanol,50% slurry; Appearance: Light blue; Ni-NTA loding density: 25~40 umol/mg gel; Protein bind capacity: 25-50 mg His-tag protein/mL settled gel; Binding target: Proteins with His-tag; ...
Form: Suspension in aqueous solution with 30% isopropanol,50% slurry; Appearance: Light blue; Ni-NTA loding density: 25~40 umol/mg gel; Protein bind capacity: 25-50 mg His-tag protein/mL settled gel; Binding target: Proteins with His-tag; ...
Stationary Phase: Ni-NTA Quantity: 10 mL Format: Suspension Format: Suspension Quantity: 10 mL 储存 The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear...