30210 凯杰Qiagen琼脂糖纯化树脂 25ml Ni-NTA Agarose ,产品介绍:产品品牌:凯杰Qiagen产品货号:30210产品名称:琼脂糖纯化树脂英文名称:Ni-NTA Agarose产品规格:25ml25 ml nickel-charged resin (max. pressure: 2.8 psi),For purification of His-tagged proteins by gravity-flow chromatography...
Principle QIAexpress Ni-NTA Protein Purification System基于获得的Ni-NTA(氨基三乙酸镍)树脂,对含有6个或以上组氨酸残基(连续或交替)亲合标签的蛋白选择能力强。该技术可在天然或变性条件下,从所有表达体系中一步纯化几乎所有的His-tagged蛋白。NTA含有四个镍离子螯合位点,与镍的结合能力比其他仅有三个金属螯合位点...
Principle QIAexpress Ni-NTA Protein Purification System基于获得专利的Ni-NTA(氨基三乙酸镍)树脂,对含有6个或以上组氨酸残基(连续或交替) 亲合标签的蛋白选择能力强。该技术可在天然或变性条件下,从所有表达体系中一步纯化几乎所有的His-tagged蛋白。NTA含有四个镍离子螯合 ...
Principle QIAexpressNi-NTA Protein Purification System基于获得的Ni-NTA(氨基三乙酸镍)树脂,对含有6个或以上组氨酸残基(连续或交替)亲合标签的蛋白选择能力强。该技术可在天然或变性条件下,从所有表达体系中一步纯化几乎所有的His-tagged蛋白。NTA含有四个镍离子螯合位点,与镍的结合能力比其他仅有三个金属螯合位点的...
ChristophGeisler,Donald L.Jarvis, inProtein Expression and Purification, 2018 3.1Discovery and characteristics Trichoplusia nicell line virus, orTnCLV, was first described in 2007 by a group at the National Institute of Infectious Diseases in Japan[29]. Much like the discovery of Sf-rhabdovirus at...
□ Protein Purification Principle His60 Ni Protein Purification Resin, which is based on a modified Ni-IDA resin, provides remarkable selectivity and reliability when is it used to purify proteins containing an affinity tag of six or more consecutive histidine residues―such as the 6xHis tag...
For the PPI assay, Ni-NTA magnetic agarose beads were used to bind the PPI complex with His6-tag. Principle of the assay In the assay, GST–tagged LEDGF/p75 IBD (yellow) is mixed with His6-tagged IN CCD (green) at the desired concentrations. After incubation at room temperature, a ...
which increases the workload of purification and impurity removal. Compared with the above two methods of removing Cu, the Na2S2O3method has more potential for industrial large-scale application; the principle applied is that in a neutral or acidic environment, Na2S2O3and Cu2+can undergo complexation...
In this context, ethylenediamine disuccinic acid (EDDS), nitrilotriacetic acid (NTA), hydroxy ethylene diamine triacetic acid (HEDTA), and ethylenediaminetetraacetic acid (EDTA) were extensively used for the enhanced phytoextraction of Ni [1,15]. However, several limitations in the use of these ...
4.5. Separation and Purification of PMI The crude enzyme solution was filtered by microporous membrane (0.45 µm) and purified by Co-NTA gravity affinity chromatography (Equilibrium buffer: 1 × PBS, NaCl 0.5 mol·L−1, imidazole 20 mmol·L−1, pH 7.4. Elution buffer: 1 × PBS, ...