The article presents a study which examines the products of expression of HydSL nickel (Ni)-iron (Fe) hydrogenase structural genes obtained from the purple sulfur bacterium Thiocapsa roseopersicina in Escherichia coli. The study aims to create genetic constructs which contain the structural genes ...
Density functional theory (DFT) was employed to investigate the behavior of a series of catalysts used in the hydrogen evolution reaction (HER, 2H(+) + 2e(-) --> H(2)). The kinetics of the HER was studied on the [NiFe] hydrogenase, the [Ni(PS3*)(CO)](1)(-) and ...
HYDROGENASEOXIDATION-reduction reactionDENSITY functionalsWe have applied density functional theory, using both pure (BP86) and hybrid (B3LYP and B3LYP*) functionals, to investigate structural parameters and reaction energies for nickel(II)-sulfur coordination compounds, as well a...
The soluble NAD+-reducing Ni−Fe hydrogenase (SH) from Ralstonia eutropha H16 is remarkable because it cleaves hydrogen in the presence of dioxygen at a unique Ni−Fe active site (Burgdorf et al. (2005) J. Am. Chem. Soc. 127, 576). By X-ray absorption (XAS), FTIR, and EPR ...
This residue forms a hydrogen bond with the spin-carrying Ni-coordinated sulfur of Cys-549. Comparison with other hydrogenases reveals that the active site is essentially the same in all proteins, including a regulatory hydrogenase.doi:10.1007/s00775-004-0613-5Stefanie Foerster...
flavidum hydrogenase as well as the cloning and sequencing of the genes encoding it. Findings of biochemical analysis of soluble hydrogenase from the thermophilic bacterium, acetomicrobium flavidum; Most important biochemical properties of enzyme; Enzyme's stability to oxygen inactivity.Mura...
Shirshikova GN, Khusnutdinova AN, Postnikova OA, Patrusheva EV, Butanaev AM, et al. Expression of Ni-Fe hydrogenase structural genes derived from Thiocapsa roseopersicina in Escherichia coli . Doklady Biochemistry and Biophysics. 2009; 425 :124–126....
In the Ni-A state, the enzyme is inhibited and cannot turn over molecular hydrogen. Ideally, if the Ni-A state can be avoided, and if the oxidized enzyme exclusively resides in the "ready" Ni-B state, it can be quickly activated, thus increasing its activity of hydrogen production. In ...
B.; Reijerse, E.; Lubitz, W. Models of the Ni-L and Ni-Sla States of the NiFe -Hydrogenase Active Site. Inorg. Chem. 2016, 55, 419-431; (b) Perotto, C. U.; Marshall, G.; Jones, G. J.; Stephen Davies, E.; Lewis, W.; McMaster, J.; Schroder, M. A Ni(I)Fe(II)...
Sellmann D,Moll G F.[Ni(NHPnPr3)(‘S3‘)], the First Nickel Thiolate Complex Modeling the Nickel Cysteinate Site and Reactivity of [NiFe] Hydrogenase.Angewandte Chemie International Edition.D. Sellmann,F. Geipel,M. Moll.[Ni(NHPnPr3)(‘S3‘)], the First Nickel Thiolate Comp...