2. NHS Ester基团:NHS Ester是琥珀酸酯基团(NHS Acetyl group)的衍生物,常用于生物偶联和活化末端制备。它提供了一个可以与目标分子或生物大分子发生反应的活性酯基。 3. 螯合基团:螯合基团是决定螯合剂性能的关键部分。NOTA-NHS Ester作为一种双功能螯合剂,具有两个功能基团,一是与金属离子配位的螯合基,二是与...
一、试剂基团反应特点(Reagent group reaction characteristics):DusQ 2琥珀酰亚胺酯是一种具有胺反应性基团的衍生物,用于用胺修饰的寡核苷酸进行标记,含有胺的蛋白质和其他分子。DusQ 2是一种无荧光猝灭剂,吸光度为552纳米。它可用作荧光共振能量转移(FRET)应用中的受体与黄色和橙色至红色发射染料结合,例如Cy...
【性质简介】:The NHS ester group, as an active group, has a unique ability to react with compounds containing amino groups. This reaction can form stable amide bonds, providing a solid foundation for the connection between biomolecules. In biological coupling reactions, Bis-PEG25-NHS ester reagen...
ICG NHS ester is a product that has the most representative NHS ester reactive group for labeling amine. Because it rapidly and high-specifically reacts to Primary amine (-NH2), it can label Protein containing amine (-NH2), Antibody and Amine-modified Oligonucleotide in a short time period. ...
ICG NHS ester is a product that has the most representative NHS ester reactive group for labeling amine. Because it rapidly and high-specifically reacts to Primary amine (-NH2), it can label Protein containing amine (-NH2), Antibody and Amine-modified Oligonucleotide in a short time period. ...
一、试剂基团反应特点(Reagent group reaction characteristics):Sulfo-Cy5(Me) NHS ester(磺酸基-Cy5-N-羟基琥珀酰亚胺酯)的反应机理主要涉及与生物分子中的氨基(NH2)发生共价酰胺化反应,从而实现对生物分子的荧光标记。以下是该反应机理的详细说明:1.活化:首先,Sulfo-Cy5(Me) NHS ester中的NHS酯(N-...
一、试剂基团反应特点(Reagent group reaction characteristics):TCO NHS ester(反式环辛烯-琥珀酰亚胺酯)的反应原理主要涉及其与氨基的反应,生成稳定的酰胺键。TCO-NHS(轴向)是合成TCO接头的基础,TCO将通过反电子需求Diels-Alder环加成反应与四嗪反应,形成稳定的二氢哒嗪键。这种反应具有选择性和生物相容性,...
金属原子或离子与含有两个或连两个以上配位原子的配位体作用,生成具有环状结构的络合物,该络合物叫做螯合物。能生成螯合物的这种配体物质叫螯合剂,也称为络合剂。又称螯合配体(chelating ligand),螯合基团(chelating group)或多齿配体(mutidentate ligand)。配体中有两个或两个以上配位原子,且同时与一个中心...
groups. in the modification and labeling process of biomolecules, the primary amine group of proteins often plays a crucial role, and nhs esters can react specifically with it to achieve precise labeling of proteins. in addition, the nhs ester group of bis-peg9-nhs ester can also react with...
Cy5 NHS ester,Cy5 NHS ester(Cyine5-NHS)是一种红色荧光染料,用于标记肽、蛋白质和寡核苷酸中的氨基。