This labeling protocol was developed for the conjugate of Goat anti-mouse IgG with ATTO 590 NHS ester. You might need further optimization for your particular proteins. Note:Each protein requires a distinct dye/protein ratio, which also depends on the properties of dyes. Over-labeling of a prot...
We recommend using the following general protocol for the labeling of biomolecules with NHS esters produced byLumiprobe. Calculate required amount of NHS ester:NHS_ester_weight [mg] = 8 × amino_compound_weight [mg] × NHS_ester_molar_weight [Da] / amino_compound_molar_weight [Da].8 is mo...
Labeling is performed according to the standard protocol from GE Healthcare. 50 µg of protein extract was labeled on ice in the dark for 30 min with 400 pmoles of the N-hydroxysuccinimide esters of the Z-CyDyes (Z-Cy2, Z-Cy3 and Z-Cy5) dissolved in 99.8% DMF. Labeling reactions ...
UV-Tracer™ TCO-NHS ester UV-Tracer™ Trans-Cyclooctene-NHS ester for Labeling of primary Amine-groupsd = layer thickness of cuvette (e.g. 1 cm)A350 = Protein absorbance at 350 nmε350 = molar extinction coefficient of UV-TracerTM TCO- NHS ester=19500 M-1cm-1A280 = protein ...
Cy3® NHS Ester (GE Healthcare) and Cyanine3 monosuccinimidyl ester are chemically equivalent, reactive dyes used for fluorescence labeling biological molecules by targeting amino-groups. Background Cy3 is a yellow fluorescent dye belonging to the cyanine dyes family. It is sometimes also referred ...
We recommend using the following generalprotocol for the labeling of biomolecules with NHS esters produced by Ruixibio. 1. Calculate required amount of NHS ester: NHS_ester_weight [mg] = 8 ×amino_compound_weight [mg] × NHS_ester_molar_weight [Da] / ...
Cy5.5-NHS ester is a near-infrared amine reactive dye for labeling amino groups of peptides, proteins and oligonucleotides. Cas No. 1469277-96-0 SDF 别名 CY5.5N-羟基琥珀酰亚胺酯 化学名 3-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E,5E)-5-(1,1,3...
• Specific—NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)• Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labelingApplications:• Primary ...
• Specific—NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)• Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labelingApplications:• Primary ...
A DNP moiety containing a PEG linker and an NHS ester terminal group which can be used for labeling biomolecules with a DNP hapten tag.