Yields were highest using a 3: 1 epichlorohydrin to NH4OH molar ratio, producing weak anion exchangers. The weak anon exchangers had low dye-binding capacities compared to their total anion-exchange capacities. A 2 : 1 epichlorohydrin-to-imidazole molar ratio proved best for making strong ani...
Although NaOH creates an alkali condition that improves protein solubility and yield, NaOH is a strong alkali and also causes degradation [34]. Therefore, a mild alkaline application with less severity is needed. NH4OH is a weak base that creates a mild alkali processing condition, which could ...