研究对象进行NGS检测使用的是Illumina TruSight sequencing panel。其中血液恶性肿瘤panel共包含54个基因,涵盖与血液恶性肿瘤报告相关的最具临床意义的突变。这些基因包括: ABL1、ASXL1、ATRX、BCOR、BCORL1、BRAF、CALR、CBL、CBLB、CBLC、CDKN2A...
Newly diagnosed isolated myeloid sarcoma–paired NGS panel analysis of extramedullary tumor and bone marrowMyeloid sarcomaCHIPClonal evolutionNGS sequencingHomingIsolated myeloid sarcoma (MS) is a rare malignancy in which myeloid blast forms tumors at various locations while the bone marrow (BM) remains ...
A cohort of 112 patients diagnosed with MN according to the revised fourth edition of the WHO classification (WHO-HAEM4R) underwent testing with a 141-gene NGS panel for hematological diseases. Ancillary studies were also conducted, including bone marrow cytomorphology and routine cytogenetics. The...
Mutational load correlated with degree of bone marrow infiltration: BM samples with no mutation had the lowest plasma cell infiltration: mean 1.6% by MFC (range, 0.003 - 11.7%), 20% by CM (1 - 90%), and 24% by HP (1 - 80%). Presence of at least one mutation/sample corresponded ...
Precision ID DL8 Kit, IonCode Barcode Adapters, and GlobalFiler NGS STR Panel v2 Where can I find the sequences for the IonCode barcodes? The sequences for the barcodes can be found in the Barcodes section of ...
Serum,WB,Respiratory,CSFStool,Tissue,BoneMarrow 1hour 1hour SequenceGeneration 12-40hours cDNASynthesis1)RNA(DNase) 2)DNA(methyldepletion) 2-4hours 2-3hours TOTALTURNAROUND TIME:48-72hours P Pre-Treatment (Beadbeating) Li LibraryPreparationandQualityControl ...
QBDA AML panel was applied to clinical samples, and was compared with other MRD detection methods including MFC12and conventional NGS28. Ten paired bone marrow aspirates from five AML patients sampled at diagnosis and during complete remission were tested by QBDA panel. All patients chosen wereNP...
A bone marrow MRD evaluation was planned before and after maintenance for all patients achieving at least very good partial response (VGPR). A one-mL bone marrow aspirate was sent overnight to one of the central labs. The primary purpose was to assess MRD by FCM. When extra cells were ...
A cohort of 112 patients diagnosed with MN according to the revised fourth edition of the WHO classification (WHO-HAEM4R) underwent testing with a 141-gene NGS panel for hematological diseases. Ancillary studies were also conducted, including bone marrow cytomorphology and routine cytogenetics. The...
The MyMRD gene panel is a sensitive and reliable assay that provides monitoring of MRD using genomic DNA and cfDNA. The assay is shown to detect clinically important driver variants and to have excellent linearity and LoD for targeted sites. The assay provides an accurate method for detecting ...