Human neurons engineered from induced pluripotent stem cells (iPSCs) through neurogenin 2 (NGN2) overexpression are widely used to study neuronal differentiation mechanisms and to model neurological diseases. However, the differentiation paths and heterogeneity of emerged neurons have not been fully ...
cells within the developing central and peripheral nervous systems. NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord ...
2021年6月24日,国际著名学术杂志Cell 子刊Stem Cell Reports杂志在线发表了华中科技大学基础医学院张斌课题组题为Reprogramming astrocytes to motor neurons by activation of endogenous Ngn2 and Isl1的研究论文。 中枢神经系统损伤和神经退行性疾病会导致不可逆的神经元丢失。过表达内源性特异的转录因子可以将体细胞重...
cells within the developing central and peripheral nervous systems. NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord ...
材料和方法部分: Ngn2 was a gift from Malin Parmar (Addgene plasmid # 34999) 参考文献部分: Direct conversion of human fibroblasts to dopaminergic neurons. Pfisterer U, Kirkeby A, Torper O, Wood J, Nelander J, Dufour A, Bjorklund A, Lindvall O, Jakobsson J, Parmar M. Proc Natl Acad Sc...
Supplementary Figure 10: Generation of iNeurons by NGN2 activation. From: Highly efficient Cas9-mediated transcriptional programming Bright field images of dCas9-AD NGN2 mediated iNeurons, four days after doxycycline addition. Red arrows point towards the cell body of iNeurons. Scale bar represents...
NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord injuries. Handling: The antibody solution should be gently mixed before ...
) transcription factor that can specify a neuronal fate on ectodermal cells and is expressed in neural progenitor cells within the developing central and peripheral nervous systems The protein product of this gene also plays a role in the differentiation and survival of midbrain dopaminergic neurons...
We describe a CRISPR inhibition (CRISPRi) protocol to repress endogenous gene expression (e.g., ATP6V1A) in human induced pluripotent stem cell-derived NGN2-induced glutamatergic neurons. CRISPRi enables efficient and precise gene repression of one or multiple target genes via delivering gRNA(s)...
Adult tissue stem cells contribute to tissue homeostasis and repair but the long-lived neurons in the human adult cerebral cortex are not replaced, despite evidence for a limited regenerative response. However, the adult cortex contains a population of proliferating oligodendrocyte...