Adult tissue stem cells contribute to tissue homeostasis and repair but the long-lived neurons in the human adult cerebral cortex are not replaced, despite evidence for a limited regenerative response. However, the adult cortex contains a population of proliferating oligodendrocyte...
cells within the developing central and peripheral nervous systems. NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord ...
材料和方法部分: Ngn2 was a gift from Malin Parmar (Addgene plasmid # 34999) 参考文献部分: Direct conversion of human fibroblasts to dopaminergic neurons. Pfisterer U, Kirkeby A, Torper O, Wood J, Nelander J, Dufour A, Bjorklund A, Lindvall O, Jakobsson J, Parmar M. Proc Natl Acad Sc...
Decreases in specific brain markers, such as emx1 in the telencephalon, ngn1 in differentiated neurons, and otx2 in the midbrain, were observed in ... HC Chen,LC Hsu,JL Wu,... - 《Environmental Toxicology》 被引量: 12发表: 2014年 加载更多来源...
NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord injuries. Handling: The antibody solution should be gently mixed before ...
Neurogenin 2 (NGN2), a neuronal transcription factor, can expedite differentiation of stem cells into mature glutamatergic neurons. We have utilized an allelic series of previously published and characterized isogenic Huntington's disease (IsoHD) human embryonic stem cell lines (Ooi et al., 2019),...
) transcription factor that can specify a neuronal fate on ectodermal cells and is expressed in neural progenitor cells within the developing central and peripheral nervous systems The protein product of this gene also plays a role in the differentiation and survival of midbrain dopaminergic neurons...
However, overexpression of Cdkn1c induces cell cycle exit and restores the generation of neurons. These findings highlight that cell type conversion can occur through two distinct mechanistic paths, even when induced by closely related transcription factors. Expression of transcription factors can ...
Coupled with genome editing and/or patient iPS cell lines, Ngn2-iN cells provide an excellent experimental system that can harness rapid, reliable, and renewable source of human neurons in a culture dish. Here, we describe a stepwise protocol in generating Ngn2-iN cells from both feeder-free...
In the Ngn2 group and Nrf2/HO-1 group, the neurons on the injured side were improved, and the loss, disorder, and adhesion of nerve cells were still observed. In the combined group, the necrosis of nerve cells was reduced and cell infiltration was improved. (3) Compare...