In the Ngn2 group and Nrf2/HO-1 group, the neurons on the injured side were improved, and the loss, disorder, and adhesion of nerve cells were still observed. In the combined group, the necrosis of nerve cells was reduced and cell infiltration was improved. (3) Compare...
The role of TRPM2 channels in neurons, 调节神经胶质细胞活性以及 Nrf2/HO-1 等蛋白因子的表达相 关.但研究依然存在一定局限性,对 Ngn2 通过何种方式调 glial cells and the blood-brain barrier in cerebral ischemia and hypoxia. Acta Pharmacol Sin. 2018;39(5):713-721. [18] WANG J, ISHFAQ M, ...
cells within the developing central and peripheral nervous systems. NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord ...
Here, we describe a workflow to engineer a doxycycline-inducible NGN2 (neurogenin 2) cassette into the AAVS1 (adeno-associated virus integration site 1) locus and differentiate positive clones into neurons. iPSCs are electroporated with ribonucleoprotein and a donor plasmid. The positive clone ...
NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord injuries. Handling: The antibody solution should be gently mixed before ...
Supplementary Figure 10: Generation of iNeurons by NGN2 activation. From: Highly efficient Cas9-mediated transcriptional programming Bright field images of dCas9-AD NGN2 mediated iNeurons, four days after doxycycline addition. Red arrows point towards the cell body of iNeurons. Scale bar represents...
However, despite these similarities, neurons constitute the m... N Bertrand,DS Castro,F Guillemot - 《Nature Reiview Neuroscience》 被引量: 2412发表: 2002年 Primary neurogenesis in Xenopus embryos regulated by a homologue of the Drosophila neurogenic... Examines the function of the Drosophila ...
Coupled with genome editing and/or patient iPS cell lines, Ngn2-iN cells provide an excellent experimental system that can harness rapid, reliable, and renewable source of human neurons in a culture dish. Here, we describe a stepwise protocol in generating Ngn2-iN cells from both feeder-free...
Adult tissue stem cells contribute to tissue homeostasis and repair but the long-lived neurons in the human adult cerebral cortex are not replaced, despite evidence for a limited regenerative response. However, the adult cortex contains a population of proliferating oligodendrocyte progenitor cells (OPC...
We describe a CRISPR inhibition (CRISPRi) protocol to repress endogenous gene expression (e.g., ATP6V1A) in human induced pluripotent stem cell-derived NGN2-induced glutamatergic neurons. CRISPRi enables efficient and precise gene repression of one or multiple target genes via delivering gRNA(s)...