In the Ngn2 group and Nrf2/HO-1 group, the neurons on the injured side were improved, and the loss, disorder, and adhesion of nerve cells were still observed. In the combined group, the necrosis of nerve cells was reduced and cell infiltration was improved. (3) Compare...
Ascl1 and Ngn2, closely related proneural transcription factors, are able to convert mouse embryonic stem cells into induced neurons. Despite their similarities, these factors elicit only partially overlapping transcriptional programs, and it remains unknown whether cells are converted via distinct mechanis...
cells within the developing central and peripheral nervous systems. NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord ...
NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord injuries. Handling: The antibody solution should be gently mixed before ...
Here, we describe a workflow to engineer a doxycycline-inducible NGN2 (neurogenin 2) cassette into the AAVS1 (adeno-associated virus integration site 1) locus and differentiate positive clones into neurons. iPSCs are electroporated with ribonucleoprotein and a donor plasmid. The positive clone ...
Supplementary Figure 10: Generation of iNeurons by NGN2 activation. From: Highly efficient Cas9-mediated transcriptional programming Bright field images of dCas9-AD NGN2 mediated iNeurons, four days after doxycycline addition. Red arrows point towards the cell body of iNeurons. Scale bar represents...
1. Intracellular recordings were obtained from neurons in parasagittal brain slices of the guinea pig ventral cochlear nucleus (VCN). The principal neurons... JA White - 《Journal of Neurophysiology》 被引量: 50发表: 1994年 SO SNH NNG SUT SINH SN CA LN NI VCN-MS15 VI LN NI MNG CI NUI...
Adult tissue stem cells contribute to tissue homeostasis and repair but the long-lived neurons in the human adult cerebral cortex are not replaced, despite evidence for a limited regenerative response. However, the adult cortex contains a population of proliferating oligodendrocyte progenitor cells (OPC...
Overexpression of NGN2 in human induced pluripotent stem cells (hiPSCs) or human embryonic stem cells has been shown to efficiently trigger conversion to neurons. Here we describe two gene-edited hiPSC lines harbouring a doxycycline (DOX)-inducible cassette in the AAVS1 locus driving expression of...
However, overexpression of Cdkn1c induces cell cycle exit and restores the generation of neurons. These findings highlight that cell type conversion can occur through two distinct mechanistic paths, even when induced by closely related transcription factors. Expression of transcription factors can ...