Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect cu...
基因转染目的探讨Ngn2基因对小鼠体外培养三维视杯结构视网膜神经元分化的调控作用.方法采用小鼠诱导多能干细胞(iPSC)在特定条件下体外培养获取视泡结构(OV),OV培养过程中通过慢病毒介导Ngn2基因多次转染OV,并在OV发育成熟后进行诱导,促进OV内细胞向视网膜神经细胞的特异性分化.以绿色荧光蛋白(EGFP)基因作为对照,免疫...
CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived. Neurons Neuron 104, 239–255.e12 (2019). CAS PubMed Google Scholar Tracey, T. J., Steyn, F. J., Wolvetang, E. J. & Ngo, S. T. Neuronal lipid metabolism: multiple pathways driving functional ...
CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived neurons Neuron, 104 (2019), pp. 239-255.e12 View PDFView articleView in ScopusGoogle Scholar Topol et al., 2015 A. Topol, N.N. Tran, K.J. Brennand A guide to generating and using hiPSC derived NPCs...