Neuron heterogeneity is observed across multiple iPSC clones and lines from different individuals. We find that neuron fate acquisition is sensitive to NGN2 expression level and the duration of NGN2-forced expression. Our data reveal that NGN2 dosage can regulate neuron fate acquisition, and that ...
基因转染目的探讨Ngn2基因对小鼠体外培养三维视杯结构视网膜神经元分化的调控作用.方法采用小鼠诱导多能干细胞(iPSC)在特定条件下体外培养获取视泡结构(OV),OV培养过程中通过慢病毒介导Ngn2基因多次转染OV,并在OV发育成熟后进行诱导,促进OV内细胞向视网膜神经细胞的特异性分化.以绿色荧光蛋白(EGFP)基因作为对照,免疫...
Development of a fully human assay combining NGN2-inducible neurons co-cultured with iPSC-derived astrocytes amenable for electrophysiological studiesNeurogenin 2 encodes a neural-specific transcription factor (NGN2) able to drive neuronal fate on somatic and stem cells. NGN2 is expressed in neural ...
Generation of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locusNeurog2 is the gene encoding the neuronal transcription factor NGN2, which can convert stem cells into functional neurons in a fast and efficient way. Here we ...