Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human NG2/CSPG4 protein (hCSPG4-Myc/DDK; +), using NG2/CSPG4 Antibody (upper), DYKDDDDK Tag Antibody #2368 (middle), and α-Actinin (D6F6)...
One cell type that contributes to this lineages is the pericyte, a cell expressing Ng2/Cspg4. Using lineage tracing, we demonstrated that bone and soft tissue sarcomas driven by the deletion of the Trp53 tumor suppressor, or desmoid tumors driven by a mutation in Apc, can derive from ...
Serum response factor: toggling between disparate programs of gene expression. J. Mol. Cell Cardiol. 35, 577–593 (2003). Article CAS Google Scholar Chen, J. et al. CD146 coordinates brain endothelial cell-pericyte communication for blood-brain barrier development. Proc. Natl. Acad. Sci. ...
To further examine pericyte labeling by tdTomato, we stained tissue sections for another SMC/pericyte marker, chondroitin sulfate proteoglycan 4 (CSPG4; also named as NG2). However, tdTomato was not detected in NG2+ cells (Figure 1C). In addition, no tdTomato+ SMCs were identified by ...
血管生成通常从毛细血管开始,它在肿瘤生长、维持和转移中起重要作用。 肿瘤中的血管形成可以通过几个细胞过程诱导(图 1),简要总结如下。 【5】血管形成的机制。正常组织和肿瘤中的新血管形成通过以下一种或多种机制发生:a 发芽血管生成:...
PericyteretinaNG2-tdTomatoPDGFRb-tdTomatofate mappingNG2 PROTEOGLYCANIN-VIVOPDGFBRAINMICEPurpose Pericytes (PCs), located abluminal of endothelial cells on capillaries, are essential for vascular development and stability. They display a heterogeneous morphology depending on organ localization, differentiation...
Results Vhl inactivation led to a significant increase in angiogenic gene and Epo expression. This was associated with EPO﹊ndependent expansion of capillary networks in cortex, striatum and hypothalamus, as well as pericyte proliferation. A comparable phenotype resulted from the combined inactivation of...
A small subset of the YFP+ cells were found to express the pericyte marker SMA (YFP+ SMA+ cells) in the cortices of NG2CRE/iDTR/YFP mice (Figure S2A). DT administration might therefore kill these cells along with the NG2 glia, adversely affecting brain vasculature and/or the BBB. ...
To exclude that this novel mechanism is pericyte-specific, we additionally analyzed the expression of ICAM-1 in dependency of NG2 in two glioblastoma cell lines. We found that A1207 and M059K cells exhibit an inverse expression pattern of NG2 and ICAM-1. Finally, downregulation of NG2 in ...