pNiFty-SEAP plasmid is composed of three key elements: an ELAM proximal promoter, five NF-κB repeated transcription factor binding sites (TFBS) and a SEAP (Secreted alkaline phosphatase) reporter gene. Back to the top Specifications Transcription factor binding sites: NF-κB (5x) Minimal ...
TLR-signaling reporter plasmid- AmpicillinR - Luciferase pNiFty-Luc plasmid is composed of three key elements: an ELAM proximal promoter, five NF-κB repeated transcription factor binding sites (TFBS) and a Luc (Luciferase) reporter gene. Back to the top Specifications Transcription factor bi...
NFKB1 promoter/exon 1 luciferase reporter plasmid constructs containing the -94delATTG allele and transfected into either HeLa or HT-29 cell lines showed less promoter activity than comparable constructs containing the -94insATTG allele. Therefore, we have identified the first potentially functional ...
(d) U2OS cells were transiently co- transfected with an NF-kB reporter plasmid and an siRNA pool targeting ARTD10 mRNA. The serum-starved cells were stimulated with TNFa before the analysis of luciferase activity, which was standardized to b-Gal. Error bars represent s.d. (n ¼ 6), *...
5、.cornABSTRACT:ObjectiveToelvaluatetheeffectoflowsalt(LS)ontheexpressionofcyclooxygenase-2(COX-2)andtheactivityofnuclearfactorkappaB(NFKs)andactivatorprotein一1(AP一1)inthemousemaculadensaderived(MMDDI)cellline.MethodsMMDDIcellsweretransfectedwithluciferasereporterplasmidcontainingAP一1orNFKB.Luciferase 6、...
a The luciferase reporter assay using a STAT3-Luc (a) reporter plasmids. H. pylori infection of AGS-pcDNA cells significantly increased the luciferase activity, which was reduced after treatment with BAY (10 µM) or transfection with Iκ-B super-repressor plasmid The bar graphs ...
Drosophila S2 cells were transfected with 0.05 mg of protein expression vector (LvIKKb, LvIKKe1 or LvIKKe2), 0.05 mg luciferase reporter plasmid (pGL3-Basic, pGL3-PEN453, pGL3-PEN309, pGL3-PEN4, pGL3-Drs, or pGL3-AttA) and 0.005 mg pRL-TK Renilla luciferase plasmid (as an internal ...
Functional activity of this cell line has been validated by transfecting the MD2 plasmid and analyzing SEAP activity with IMGENEX�s SEAPorter� Assay Kit (10055K) (Fig. 2). IMGENEX is pleased to offer the TLR4/NF-kB Reporter Assay as a service. Complete Growth Medium(*...
Furthermore, by using transgenic mice possessing an NF-κB-responsive lacZ reporter gene, the responses of mouse host cells to H. pylori infection were investigated in vivo. It was suggested that H. pylori may be able to regulate NF-κB signaling during chronic infection [14]. However the ...
Each PCR amplified DNA was cloned into the pFBGR plasmid (a pFastBac™ variant obtained from Dr. Robert Kotin, NIH/NHLBI), a plasmid containing two AAV inverted terminal repeats (ITRs) flanking the CMV promoter and EGFP reporter. The EGFP is separated (not fused) from PARP-1 gene by a...