相比之下,在40号分箱中,未刺激的细胞中Brg1 ChIP-seq峰值仅在0.8%的ATAC-seq峰值区域被观察到,但在刺激后这一比例上升到37%。 针对p300(一种转录共激活因子)和组蛋H3K27ac(一种与活跃转录相关的组蛋白修饰),也得到了类似的结...
接着,作者使用ChIP-seq方法寻找JunB的直接作用靶点。结果显示在5号染色体上基因SQSTM1的转录起始位点(TSS)上游1.8kB有一个结合峰,SQSTM1编码一个对NF-kB激活和自噬非常重要的蛋白。同时在该基因的启动子区域富集了H3K27Ac修饰,它是活性增强子...
Applications: WB, IHC, IP, ChIP-seq Reactivity: Hu, Ms Conjugate/Tag: Unconjugated Quantity: 100 µg Citations: (11) Figures: (14) Supplier Page USD433.00 Compare Product Rabbit anti-RelA Antibody, Affinity Purified Applications: Immunoprecipitation (IP) Reactivity: Human (Hu) Conjugate...
ChIP-Seq (chromatin immunoprecipitation with massively parallel DNA sequencing) and CUT&RUN (cleavage under target and release using nuclease) assays have been employed to further analyze the functional role of canonical Rela pathway members. By interrogating these mechanisms, novel therapeutic targets ...
ChIP-Seq analyses show that RelA directly binds to the majority of Treg signature genes, with a significant overlap with Foxp3-bound loci (Heuser et al., 2017; Oh et al., 2017). This cooperation between Foxp3 and NF-κB was documented at the GITR locus (Tone et al., 2014). NF-κB...
全基因组ChIP-Seq测序发现,PRDM14与 OCT4,NANOG这些超级转录因子在控制干细胞更新和分化的基因 上具有共定位。还有研究表明PRDM1和PRDM14可以影响生殖细 胞的特化过程,PRDM9与减数分裂过程中的染色体联会有关。PRDM 家族蛋白还和血管生成,神经系统发育,癌症发生过程密切相关。 发明内容 [0006]本发明的目的在于提供一...
By employing reporter assays, EMSA, inhibition studies, bisulphite sequencing, ChIP-Seq and gene-editing we show that the p50/p50 homodimer known to act as repressor for a number of pro-inflammatory genes plays a central role in the genetic regulation of resistin in monocytes along with promoter...
高通量测序技术能检测染色体结构变异,拷贝数变异,序列信息变异等基因组水平遗传变异,也可以分析DNA甲基化,组蛋白修饰等表观遗传学状态,还可以结合RNA-seq,ChIP-seq等相关技术分析基因的表达调控.高通量测序可以一次性获得大量多维的数据,在分子生物学水平上对癌症进行全面系统的研究,用科学统计分析方法,发现肿瘤相关的...
Indeed, genome-wide p65 ChIP-seq analysis in MEFs stimulated with TNF/LPS revealed a strong peak in the promoter- proximal region of the REGg gene (Supplementary Fig. 2F). To validate in vivo binding of NFkB to the REGg promoter in colon epithelial cells, we performed ChIP assays using ...
It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.BSA and Azide Free a...