NFAT GFP Reporter Plasmid是翌圣生物自主研发的用于检测NFAT转录活性水平为目的的报告基因。NFAT(Nuclear factor of activated T-cells)转录因子家族对细胞因子基因和其它一些对免疫反应很关键的基因转录中起关键作用。 NFAT-GFP报告基因主要用于检测细胞PKC/Ca++信号通路中NFAT的转录活性、药物研究以及基因过表达和RNAi的...
NFAT GFP Reporter Plasmid是用于检测NFAT转录活性水平为目的的报告基因。NFAT(Nuclear factor of activated T-cells)转录因子家族对细胞因子基因和其它一些对免疫反应很关键的基因转录中起关键作用。 NFAT-GFP报告基因主要用于检测细胞PKC/Ca++信号通路中NFAT的转录活性、药物研究以及基因过表达和RNAi的表型分析等。
NFAT GFP Reporter Plasmid是用于检测NFAT转录活性水平为目的的报告基因。NFAT(Nuclear factor of activated T-cells)转录因子家族对细胞因子基因和其它一些对免疫反应很关键的基因转录中起关键作用。 NFAT-GFP报告基因主要用于检测细胞PKC/Ca++信号通路中NFAT的转录活性、药物研究以及基因过表达和RNAi的表型分析等。 pGMNF...
更新日期:2017/2/7 9:46:42 所在地:中国大陆 产品型号:SY0164 简单介绍:北京百奥莱博专业生产销售NFAT-GFP报告基因质粒 报告基因检测,我公司供应的细胞生物学试剂品类齐全,且具有优势价格,欢迎新老客户垂询订购。 在线询价相关标签:NFAT-GFP报告基因质粒 NFAT GFP Reporter Plasmid SY0164 ...
Finally, we tested the effect of the GFP-SPRIEIT fusion proteins on NFAT-activated gene expression in Jurkat T cells. Plasmids encoding GFP, GFP-SPRIEIT, and GFP-SPAIAIA proteins were introduced into Jurkat cells together with an NFAT-driven luciferase reporter plasmid, and a day later the ...
The expression plasmid for Nfatc2-eYFP and H2B-mCherry were kindly provided by A. Flügel and D. Lodygin23. Coding sequences for Sox10 were additionally inserted into pEF1α-IRES-GFP to produce Sox10-expressing lentiviruses. Coding sequences for Nkx2.2 and Olig2 were inserted into pCAG-...
Loss of E2 enhancer activity in Nfatc1- eGfp-Bac-DE2 tg reporter mice, showed a clear reduction in Nfatc1 expression in CD4 þ T cells as evident from reduced GFP expression both in unstimulated as well as in stimulated cells compared with that in the Nfatc1-eGfp-Bac tg reporter mice ...
3 a). When COS cells were transfected with the wild-type NFATx-GFP plasmid, the resultant NFATx-GFP protein was localized completely in the cytoplasm of transfected COS cells (Fig. 3 a, upper left panel). In contrast, X-Δ(CRI) showed nuclear distribution, whereas some fluorescence was ...
Notably, GFP-NFATc1 overexpression did not significantly alter the effect of DGG-100629 on DDIAS expression (Fig. 2f), implying that the intracellular localization of NFATc1, rather than its protein level, is crucial for DDIAS transcription. Quinoxaline derivatives have been reported to activate ...
(A) Primary keratinocytes were infected with the Ad-GFP or Ad-hNIC viruses for the indicated times. Calcipressin mRNA levels were determined by real-time RT-PCR with primers specific for the common region of the Calcipressin gene, and values were normalized for GADPH mRNA levels and expresse...