PCR反应体系50 μL,反应液成分包括25 μL Mix,上下游引物各1 μL,DNA模板1 μL,加入ddH2O补足至50 μL。PCR反应条件为95 ℃预变性3 min、95 ℃变性30 s、56~63 ℃退火45 s、72 ℃延伸45 s、41个循环;41个循环后再72 ℃延伸5 min。2%琼脂糖凝胶电泳检查PCR产物。PCR扩增产物使用3130XL测序仪进行毛...
Christakos S, Li S, De La Cruz J, Bikle DD (2019) New developments in our understanding of vitamin metabolism, action and treatment. Metabolism 98:112–120. https://doi.org/10.1016/j.metabol.2019.06.010 Article PubMed PubMed Central Google Scholar Charoenngam N, Holick MF (2020) Immu...
New developments in osteoarthritis and cartilage biology Curr. Opin. Pharmacol., 28 (2016), pp. 8-13 View PDFView articleView in ScopusGoogle Scholar [3] J.W. Bijlsma, F. Berenbaum, F.P. Lafeber Osteoarthritis: an update with relevance for clinical practice Lancet, 377 (9783) (2011), ...
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1.3 方法 1.3.1 细胞培养 将胃癌细胞株 MKN-49P 细胞培 养于含 10%胎牛血清,100 U/mL 青霉素,100 μg/mL 链霉素的 RPMI 1640 培养液中,置于 37 ℃,含 5% CO2,95%空气,湿度饱和的细胞培养箱内,取对数 生长期的细胞用于实验. 1.3.2 含药血清制备 将 SD 大鼠随机分为 2 组, 即对照组,胃复春...
This review surveys our current understanding of the role of NF-κB in the apoptotic response and focuses on many developments since this topic was last reviewed in Oncogene 4 years ago. These recent findings shed new light on the activity of NF-κB as a critical regulator of apoptosis in ...
These recent developments shed new light on the role of NF-κB in many disease conditions including tumor development, tumor progression and anticancer treatment. This is a preview of subscription content, access via your institution Access options Subscribe to this journal Receive 50 print issues ...
Moreover, recent developments made to multidimensional liquid chromatography and mass spectrometry, including the FT-Orbitrap detector technology, have significantly advanced the discovery proteomics field (8). We have used this untargeted quantitative approach to identify proteins correlating with lymph node...
This may be attributed to the recent developments in antiretroviral treatment and the competence of the HIV team in the Aegean University Medical Faculty Infectious Diseases and Clinical Microbiology Department.Guliyeva, GünelAkyol, DenizPulluku, Hüsnü...
NK-activating ligands MICA/B on the cancer cells [94]. Split hand and foot malformation 1 (SHFM1) participates in the induction of the NF-κB axis by mediating nuclear translocation of p65. The loss of SHFM1 disrupts the NF-κB axis and promotes NK potential in tumor suppression [95]. ...