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D., Fridkin, A., Perez-Pinera, P. & Lu, T. K. Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells. Mol. Cell 54, 698–710 (2014). CAS PubMed PubMed Central Google Scholar Tsai, S. Q. et al. Dimeric CRISPR RNA...
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Targeted high-throughput DNA sequencing is a primary approach for genomics and molecular diagnostics, and more recently as a readout for DNA information storage. Oligonucleotide probes used to enrich gene loci of interest have different hybridization kin
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Sanger sequencing to confirm the KRIT1 c.1664C>T mutation was performed with a set of primers specific for exon 16 (forward: 5 -gcttttcccatattaagttgttcatt; reverse: 5 -ttggttaaacagaatcttaagcatagc) using the KAPA2G Fast HS PCR kit (Kapa Biosystems, Bath, UK) and ABI PRISM® 3130xl...
integrity sha512-HfK1aMRanKHpxemaY2gqBmL04iAPOPRj7DxtNbiDOrJK+gdwkiNRVpCpUJYbUT+aZyemKN8brqTOxzCaG6ExRg== dependencies: "@babel/template" "^7.20.7" "@babel/types" "^7.21.0" "@babel/helper-hoist-variables@^7.18.6": version "7.18.6" ...
kW h−1. Zhejiang Jiangxia Tidal Experimental Power Station adopts the form of bidirectional ball bubble water turbine generator units to achieve bidirectional power generation. It is equipped with 6 units, with a single unit capacity of 500–700 kW. The last generating unit of the power ...
Single molecule sequencers suffers from higher raw data error rate, which accounts for up to 15% of incorporated bases, however, this error rate can be minimized by multiple sequencing of same template. The tSMS from Helicos Genetic analysis system was the first commercially available true single...
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