新一代测序技术的原理 Next-generation sequencing technology overview中英文 教学PPT课件.ppt,SOLiD的特点 通量大 成本低 序列短 Ligase的方式虽然能一定程度避免phasing/pre-phasing,但增加的复杂度也降低了效率 灵活性差,对小数据量测序不适用 Two-base coding 2005 2
Next Generation Sequencing Technology USD 10000 Genome in 2015 Craig Venter’s Personal Genome September, 2007 1 year USD 1 million James Watson’s Personal Genome June, 2007 USD 2 million Human Reference Genome April, 2003 >10 years to finish USD 3 billion Personal Genome 2007 3 months ~ USD...
Motivationfornewtechnologiesdrivesitsrootsnotonlyfrompotentiallycommercialusagesuchasinpersonalisedmedicine,butalsofromgovernmentsupportedprojectssuchastheHGPorthe1000genomesprojectsaimingtosequencethegenomesof1000individualsaroundtheworldwithpricetagforgenomesequencingsinglegenomessetto50,000$.•Throughput,scalability,...
2. A showcase of future plant biology: moving towards next-generation plant genetics assisted by genome sequencing and systems biology [J] . Insuk Lee Genome Biology . 2014,第5期 机译:未来植物生物学的展示:在基因组测序和系统生物学的协助下向下一代植物遗传学迈进 3. Next-Generation Sequen...
The sequence identity of the htrA locus in the Vollum and Sterne strains was verified by whole-genome sequencing. Genomic DNA was extracted as previ- ously described42,43. The PCR amplifications were performed using the Taq (Qiagen) or Expand High Fidelity (Roche) systems. The DNA sequences ...
A Toolkit for analyzing next-generation DNA Re-Sequencing data If you have any question ,contact the email: hewm2008@gmail.com / hewm2008@qq.com and also join the QQ Group : 125293663 we will send you the newest Reseqtools (iTools_CodeXX.tar.gz) for you iTools_Code20141231.tar.gz ...
Edition 1. doi: 10.1371/currents.tol.085c713acafc8711b2ff7010a4b03733.AbstractThe phenotype represents a critical interface between the genome and the envi-ronment in which organisms live and evolve. Phenotypic characters also are a rich source of biodiversity data for tree-building, and they ...
cient and economical DNA-sequencing technologies, higher-resolution analyses have become possible. Early studies are revealing distinctive patterns of DNA mutations in different tumor types (see http:// cancergenome.nih.gov/). In the not-too-distant future, the sequencing of entire cancer cell ...
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In the context of the GC-rich genome of B. pertussis, most platforms gave similar uniformity of sequence coverage, with the Ion Torrent data giving slightly more uneven coverage. In the S. aureus genome the PGM performed better. The PGM gave very biased coverage when sequencing the extremely ...