Next-generation sequencing (NGS) is a sequencing technology that offers speed, scalability and ultra-high throughput. The technology can be used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA. NGS has applications in environmental, agricultural and forensic...
二代测序也被成为高通量测序指的是现代不同的测序技术,不同于之前的Sanger测序,它的速度更快,价格也更为便宜,主要有以下几个平台: -Illumina(Solexa) sequencing - Roche 454 sequencing - Ion torrent: Proton / PGM sequencing - SOLiD sequencing 2.原理 此处主要介绍Illumina 平台的工作原理。 从本质上而言,...
enrichment. The dried-down primers reduce the number of pipetting steps and allow preparation of reproducible libraries that sequence with high uniformity. The straightforward workflow–similar to PCR–allows someone with little to no next-generation sequencing experi...
二代测序(NGS /*Next-generation Sequencing*/)是近十年来生命科学领域发展的基础技术之一。 全基因组测序(WGS/* Whole Genome Sequencing */),RAD-Seq /*Restriction site Associated DNA Sequencing */,RNA-Seq /* RNA Sequencing */,ChIP-Seq /* ChIP Sequencing */ 以及其他若干种技术已常规应用于研究重要...
DNA序列分析软件Sequencher的Next-Generation Sequencing功能 Gene Codes致力于为您带来在同行评审期刊上开发和发布的各种比对和组装算法，但让非工程师也可以使用它们。例如，GSNAP（Tom Wu，Genentech，Inc.）被认为是正确识别剪接点的算法之一。生物信息学专家可能会像这样调用该程序：如果我们有一些工具来帮助我们了解所有...
News on Next Generation Sequencing (NGS) technologies, RNA-sequencing, clinical sequencing, and sample preparation news and articles from GenomeWeb.
Epigenetics Products, Next Generation Sequencing Library Preparation Products 应用: DNA Methylation Analysis, Bisulfite Conversion, Epigenetics 试剂盒组成 本产品提供以下试剂或组分: NEB #名称组分货号储存温度数量浓度 E7140S-20 EM-seq™ Index Primer 1 (S)E7141AVIAL-201 x 0.005 ml10 µM ...
Next Generation Sequencing Library Preparation Products 产品组分信息 本产品提供以下试剂或组分: NEB #名称组分货号储存温度数量浓度 E7180S -20 NEBNext® FFPE DNA Repair Buffer E7181AVIAL -20 1 x 0.084 ml Not Applicable NEBNext® FFPE DNA Repair Mix E7182AVIAL -20 1 x 0.048 ml Not App...
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Next Generation Sequencing Library Preparation Products 试剂盒组成 本产品提供以下试剂或组分: 特性和用法 需要但不提供的材料 1X TE (10 mM Tris pH 8.0, 1 mM EDTA) 0.2 ml thin wall PCR tubes Magnetic rack/stand (NEB #S1515S; Alpaqua®#A001322 or equivalent) ...