Next-generation sequencing (NGS) technologies have been used in diverse ways to investigate various aspects of chromatin biology by identifying genomic loci that are bound by transcription factors, occupied by nucleosomes or accessible to nuclease cleavage, or loci that physically interact with remote ...
The speed, throughput, and accuracy of NGS has revolutionized genetic analysis and enabled new applications in genomic and clinical research, reproductive health, and environmental, agricultural, and forensic science. Steps in the next-generat...
Primary equipment already available in most labs. Next Generation Sequencing (NGS) Advantages High sample throughput High sensitivity Comprehensive genomic coverage Can sequence thousands of genes simultaneously Low sample input required Fast turnaround time for high sample volumes...
Conclusion This study demonstrates the exciting potential for utilisation of next generation sequencing in routine clinical practice. However it highlights the genetic heterogeneity and high frequency of novel variants with uncertain effects on gene function in DCM. This presents considerable challenges for ...
Immuno-oncology (I/O) therapies are considered to be promising anti-cancer treatments and are generating hope and excitement among researchers, clinicians, and patients. The Ion Torrent portfolio of immuno-oncology next-generation sequencing (NGS) assays enables an innovative, multi...
viruses Article Using Next Generation Sequencing to Identify and Quantify the Genetic Composition of Resistance-Breaking Commercial Isolates of Cydia pomonella Granulovirus Gianpiero Gueli Alletti, Annette J. Sauer, Birgit Weihrauch, Eva Fritsch, Karin Undorf-Spahn, Jörg T. Wennmann and Johannes A...
Next Generation Sequencing is a technology in which nucleotides are added in parallel to the copying of a DNA strand. When nucleotides are incorporated into the growing DNA strand, a signal is generated that corresponds to the nucleotide type and positio
Sequencing malaria parasites from low density infections in small amounts of dried blood is important for large-scale genomic surveillance. Here, the authors develop and validate a highly multiplexed droplet digital PCR-based amplicon deep sequencing assay and apply it to data from Zanzibar, Tanzania...
CONCLUSION: We identified a high prevalence of gene alterations using an next-generation sequencing test. Although some benefit was associated with the matched therapy, most of the patients had disease progression as the best response, indicating the limited biological potential and unclear clinical ...
Due to the extremely low pH (0.83) and high levels of inorganic metal, the number of microbial taxa identified in the community was limited. Using Sanger sequencing, the study was able to recover over 100,000 reads that assembled into over 1000 contigs (Tyson et al., 2004). Geno...