Forensic Application of Next Generation Sequencing Technology in the Typing of Y Chromosome Genetic Markers. 数据分析处理系统十分成熟.随着生物信息量的增长,传统检测技术通量低的弊端逐渐显现,推动了法医DNA分型技术的革新.近年来,二代测序(next generation sequencing,NGS)... Y Yang,R Tao,M Li,... - ...
In order to analyze and interpret next-generation sequencing (NGS) data, we formed a multi-skilled team composed of scientists with a background in biology, informatics, pharmacology, mathematics, medicine or engineering. The team met regularly once every 1-2 weeks to set tasks and worked ...
Next-generation sequencing (NGS)-based molecular tests have revolutionized the practice of medicine with the ability to personalize diagnosis, risk assessment, and treatment of patients with cancer and non-neoplastic disorders. Given the vast amounts of quantitative and complex sequencing data generated ...
The wide application of next-generation sequencing (NGS), mainly through whole genome, exome and transcriptome sequencing, provides a high-resolution and global view of the cancer genome. Coupled with powerful bioinformatics tools, NGS promises to revolu
Next-generation sequencing allows the analysis of genomes, including those representing disease states. However, the causes of most disorders are multifactorial, and systems-level approaches, including the analysis of proteomes, are required for a more comprehensive understanding. The proteome is extremely...
Next-generation sequencing allows the analysis of genomes, including those representing disease states. However, the causes of most disorders are multifactorial, and systems-level approaches, including the analysis of proteomes, are required for a more comprehensive understanding. The proteome is extremely...
2.3. Library preparation and next generation sequencing (NGS) DNA from orf virus PCR-positive fractions were pooled for each sample and submitted to the University Core DNA Services (University of Calgary, AB, Canada) for library preparation. Libraries were prepared using the NEBNext® Ultra™...
Next-generation sequencing (NGS) is used to sequence millions or billions of DNA strands in parallel. Forensic analyses require high accuracy and reproducibility, however, templates of low copy number and degraded or contaminated samples pose challenges. NGS offers some advantages to current techniques...
We developed a metagenomic next-generation sequencing (mNGS) test using cell-free DNA from body fluids to identify pathogens. The performance of mNGS testing of 182 body fluids from 160 patients with acute illness was evaluated using two sequencing platforms in comparison to microbiological tes...
Next-generation DNA sequencing STR: Short tandem repeat SNP: Single nucleotide polymorphism PCR: Polymerase chain reaction eMERGE: Electronic Medical Records and Genomics EMR: Electronic medical record HGSC-CL: Human Genome Sequencing Center Clinical Laboratory BCM: Baylor College of Medicine ...