Introduction to NGS methods and techniques Next-generation sequencing (NGS) is a technology for determining the sequence of DNA or RNA to study genetic variation associated with diseases or other biological phenomena. Introduced for comm...
Automated Sanger sequencing This process involves a mixture of techniques: bacterial cloning or PCR; template purification; labelling of DNA fragments using the chain termination method with energy transfer, dye-labelled dideoxynucleotides and a DNA polymerase; capillary electrophoresis; and fluorescence dete...
The application of next-generation sequencing (NGS) in cancer research has been instrumental in identifying genes related to cancer, improving our insight into tumor diversity, and uncovering mutations that lead to tumorigenesis. In a recentBench Tip webinar, three presenters discussed their...
Nowrousian M (2010) Next-generation sequencing techniques for eukaryotic microorganisms: sequencing-based solutions to biological problems. Eukaryot Cell 9:1300–1310 Article PubMed Central CAS PubMed Google Scholar Oinn T, Addis M, Ferris J et al (2004) Taverna: a tool for the composition an...
Recent advances in nucleic acid sequencing technologies, referred to as ‘next-generation’ sequencing (NGS), have produced a true revolution and opened new perspectives for research and diagnostic applications, owing to the high speed and throughput of data generation. So far, NGS has been applied...
The advancement of NGS and Next-NGS technologies combined with automated phenotyping techniques have accelerated the crop improvement process. NGS technology enables the generation of reference genomes and re-sequencing of related species to understand the genetic diversity, transcriptome sequencing that ...
This sequencing techniques uses reversible terminators bound to dNTPs in a cyclical method that comprises of nucleotide incorporation, fluorescence imaging and cleavage. A fluorescently-labeled terminator is imaged as a dNTP is added and then cleaved to allow incorporation of the next base. An imaging...
•Rapid(sequenceanentiregenomeinlessthanoneday)•Lowcostincomparisontotraditionaltechniques(Sangersequencing)TheserecenttechnologiesallowustosequenceDNAandRNAmuchmorequicklyandcheaplythanthepreviouslyusedSangersequencing.
GenomeWeb [online], https://www.genomeweb.com/sequencing/oxford-nanopore-presents-details-new-high-throughput-sequencer-improvements-mini (16 Sep 2014). Karow, J. Sigma-Aldrich enters co-marketing agreement with GenapSys for Genius sequencer. GenomeWeb [online], https://www.genomeweb.com/sequencing...
The remarkable power of high-throughput next-generation sequencing (NGS) combined with traditional “tried and true” biochemical techniques provides a complementary approach to study vRNA–protein interactions in a high-throughput, specific, and unbiased fashion. This has broad applications in studying ...