In this study, we have evaluated MRD 3 months after transplantation in 106 myeloma patients using a commercial next-generation sequencing (NGS) strategy (LymphoTrack®), and compared the results with next-generation flow (NGF, EuroFlow). The use of different marrow pulls and the need of ...
The samples from which these mRNA sequences are derived are described above in the section on RNA sequencing and transcript assembly (accessions GenBank:SRX092158, GenBank:SRX092159 and GenBank:SRX101672). We also obtained 60 peripheral blood mononuclear cell (PBMC) samples from rhesus macaques ...
Doctor Karl Voelkerding of University of Utah Department of Pathology said that the CAP Next-Generation Sequencing Project Team updates the requirements to adapt to NGS evolution and its translati...
Chicken-genome re-sequencingdataset used for validation purposes. The application of CornellGBS in chickens showed high performance to infer SNPs, particularly in exonic regions and microchromosomes. This approach represents a cost-effective (~US$50/sample) and powerful alternative to current genotyping ...
Detection of leukemia mutations using multigene next-generation sequencing (NGS) can potentially be used to measure residual disease. We used a targeted 28-gene NGS panel to detect mutations and different-from-normal 10-color MFC to measure MRD in AML patients before allogeneic hematopoietic stem ...
RESEARCH ARTICLE Next Generation Semiconductor Based Sequencing of the Donkey (Equus asinus) Genome Provided Comparative Sequence Data against the Horse Genome and a Few Millions of Single Nucleotide Polymorphisms a11111 Francesca Bertolini1,2, Concetta Scimone2, Claudia Geraci1, Giu...
Matías Gutiérrez explained “This is a great milestone given that it is the first time that such rapid sequencing has been carried out in the Chilean Antarctic, but also and perhaps more important is demonstrating that any Chilean scientist can study the genetics of any biological sample, anywhe...
Approximately 24 samples were polled and purified ®using QIAquick PCR Purification Kit (Quiagen). The fragments of each library were amplified by PCR using ®specific primers for sequencing in the Illumina platform. The purification of PCR reactions was performed using the Agencourt AMPure XP ...
Recently, next generation sequencing (NGS) has promoted genetic diagnoses because of the quickness and affordability of the method. To evaluate the usefulness of NGS in genetic diagnosis, we present the genetic study of RTT-like patients using different techniques based on this technology. We ...
When the researchers learned of last summer's case of mystery encephalitis, they turned to cutting-edge genomic tools to track down the cause of the woman's disease. They using rapid next-generation genomic sequencing to search the patient's spinal fluid for non-human DNA, then compared what...