doi:10.1007/s10531-023-02716-6Waltham, Nathan J.Snape, NataleVillacorta-Rath, CeciliaBurrows, DamienBiodiversity & Conservation
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The optimal detection method forRETfusion is NGS, but FISH or PCR can also be used [54]. Regarding the design of an efficient fusion search algorithm inRET, it is important to consider the following: (1) NGS based on the study of RNA is more sensitive than if only DNA is studied, and...
Fetal bovine serum (FBS) and l-glutamine were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Benzophenone-3 was purchased from Pharma Nostra (São Paulo, Brazil). Chloroquine was provided by Farmanguinhos (Fundação Oswaldo Cruz, Rio de Janeiro, Brazil). The Lanette base used...
HRESI-MS spectrum was measured with a Thermo Fisher Scientific LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). IR spectrum was measured with a Horiba FT-210 Fourier transform infrared spectrometer (Horiba, Kyoto, Japan). Cells and reagents Human gastric cancer MKN...
All probes used were labeled with the indocarbocyanine dye Cy3 (Thermo Scientific, Waltham, MA, USA). FISH was performed on white polycar- bonate filter (0.2 mm) sections with the different oligonucleotide probes, also stained with DAPI, and mounted for microscopic evaluation. The protocol was ...
(WC, OXOID, ThermoFisher Scientific, Thermo Fisher Scientific, Waltham, MA, USA). For solid media, 1.2% agar (Agar–Agar, bacteriological, Carl Roth) was added to the respective broth media. Incubation was performed as described below; for anaerobic cultures, GasPak™ EZ incubation systems (...
Purified PCR products were quantified using Pico Green (Invitrogen, Carlsbad, CA, USA) and a Victor X3 Multilabel plate reader (Perkin Elmer, Waltham, MA, USA), according to the manufacturer's protocol. Amplicons tagged by locus were pooled in equimolar amounts and submitted for high ...
Cultures were routinely maintained in Brain Heart Infusion (BHI) medium (Sigma-Aldrich, St. Louis, USA) supplemented with 10% Fetal Bovine Serum (Thermo Fisher Scientific, Waltham, USA), 50 units/ml of penicillin, 50 μg/ml of streptomycin (both from Sigma-Aldrich), and 10 μg/...