4. Conclusions In summary, we have developed the first two-photon ratiometric fluorescent probe 1 for ALP based on the naphthalene derivative. The probe displays desirable properties such as high sensitivity, high selectivity and functioning well at physiological pH. The proposed probe has been used...
A reversed phase Agilent TC C18 column (150 cm × 4.6 mm, pore size 5 µm; Agilent Technologies, Santa Clara, CA, USA) was used. A mixture of acetonitrile −25 mM phosphate buffer pH 3 (27:73, v/v) was used as the mobile phase at a flow rate of 1.0 mL/min and the ...