The LC/MS/MS analyses used in PAMPA and metabolic stability assays were obtained on Waters ACQUITY™ TQD system (Waters, Milford, CT, USA). 3.6. Statistical Analyses The ED50 or TD50 values for tested substances in the acute animal models of seizures were calculated using the computer log-...
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All data are collected in multi-channel analysis mode and processed using Mass Lynx version 4.1 software with Target Lynx version 4.1 programme (Waters Corp., Milford, MA). One of the specific objectives of this study was to test whether IL-1b can regulate the kynurenine pathway (see Figure...
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ALA and PBG in the cerebrospinal fluid were measured by LC coupled to a triple-quadrupole MS system (Xevo TQMS, Waters, Milford, MA, USA), following a metabolite derivatization process (AccQTag, Waters, Milford, MA, USA). Porphyrins were quantified in a PerkinElmer LS50B spectrofluorometer (...
data are collected in multi-channel analysis mode and processed using Mass Lynx version 4.1 software with Target Lynx version 4.1 programme (Waters Corp., Milford, MA). Drugs All drugs and reagents were purchased from Sigma-Aldrich (St Louis, MO) unless otherwise stated. IL-1βand growth ...