Cells exposed to 1% DMSO (as vehicle) served as the control with 100% survival. Cells treated with 1 µM staurosporine served as the internal control of the cytotoxicity experiments (the viability of cancer cells was reduced to 44–49%, data not shown). Table 2. The IC50 values (µM...
RNAs isolated from the roots, stems, petioles, and leaf blades were reverse transcribed and used as template for PCR amplification of CK HKs (A) and HPts (B) as indicated. The expression profile of Clathrin (Clat) was used as control expression gene (C). 3. Discussion In the context ...
dPCR reaction was carried out in a final volume of 18 µL; this reaction included 8.55 µL of template cfDNA, 9 µL of 20X QuantStudio® Master Mix, and 0.45 µL 40X TaqMan assay. Subsequently, 14.5 µL of final reaction volume was loaded to QuantStudio® 3D digital PCR ...