City Maps out a New Way to Help Property Hunters; Ipswich Pioneers Online System to Provide Planning Information
Maps Show New Rollout for the NBN; 6100 Ipswich Households Now Being Connected to Fast Broadband ServiceByline: Joel Gould joel.gould@qt.com.au
Specific primers were designed to amplify two copies of each Opuntia-derived geminivirus that were cloned in tandem to the binary vector pJL-89 [52], excluding the 35S promoter region of the vector, using the Gibson assembly [53] (New England Biolabs, Ipswich, MA, USA), as previously ...
The collected extracts were then evaporated using a miVac QUATTRO centrifugal vacuum concentrator (SP Scientific, Ipswich, UK) to the dry residue and depending on the bioassay (antibacterial, enzyme inhibition, cytotoxicity, or acute toxicity) (see methodology below) prepared for the first testing ...
(New England BioLabs Inc., Ipswich, MA, USA) according to the manufacturer’s protocol. Briefly, poly-A RNAs were captured by oligo-dT conjugated magnetic beads and the mRNAs were eluted and fragmented at 94 °C. First-strand cDNA was generated by random priming reverse transcription, and, ...