New England Biolabs (NEB), a leading pioneer of the biotechnology industry, is a manufacturer of restriction enzymes for genomic research. The three-storey, 208,000ft² (19,324m²) built-to-suit research and production laboratories are located in the rural setting of Ipswich, Massachusetts, ...
The first library, which contained “short” fragments of genomic DNA (~400 bp) was constructed using NEBNext®DNA Library Prep Master Mix Set for Illumina® (New England Biolabs, Ipswich, MA, USA). The second and third libraries, which contained “long” genomic DNA fragments (~5 kbp ...
(New England BioLabs Inc., Ipswich, MA, USA) according to the manufacturer’s protocol. Briefly, poly-A RNAs were captured by oligo-dT conjugated magnetic beads and the mRNAs were eluted and fragmented at 94 °C. First-strand cDNA was generated by random priming reverse transcription, and, ...
1, Anna Sai 1, Valentina Burskaia 2, Ilya Artyushin 3and German Shipulin 1 1 Federal State Budgetary Institution “Centre for Strategic Planning and Management of Biomedical Health Risks” of the Federal Medical Biological Agency, 119121 Moscow, Russia ...