New England Basset Hound Rescue New England Belt Sander Racing Association New England Bible College New England Bibliographic Instruction Committee New England Biolabs Foundation New England Biolabs, Inc. New England Blue Grass Band New England Board of Higher Education ...
NEO resistant ES cell clones were checked for homologous recombination by a southern blot using a 32P labeled 5’ external probe (598 pb) used after ApaI (New England Biolabs) DNA digestion, and 3’ external probe (752 pb) used after BamH1 (New England Biolabs) DNA digestion (Supplementary...
Biochemicals and commercial enzymes were obtained from Thermo Fisher Scientific (Waltham, MA, USA) and New England Biolabs GmbH (Frankfurt am Main, Germany). Gel and plasmid purification kits were procured from Machery Nagel (Düren, Germany). A list of reagents and catalog numbers is supplied ...
proposal that does not meet the criteria of the foundation.Analysis of unsuccessful attempts, such as the Sarah Scaife Foundation, New England Biolabs Foundation, and the International Bank of Japan Foundation, point out the need for attention to the details of the foundation's procedures and ...
coli cell paste by two passes through a Microfluidizer at 10,000 psi, and captured onto chitin resin (New England BioLabs) from clarified lysate supernatant. Chitin resin was thoroughly washed prior to the overnight room temperature incubation with 2 mM C[K-fluorescein] in 200 mM ...
primers encoding BsaI cut sites that would remove the intervening region and allow for integration of the spacer between the barcode and the TtgR variant region. The spacer was inserted into the backbone using Golden Gate (New England Biolabs, BsaI-HFv2, E1601L) following manufacturer protocols....
The recombinant Cas9 protein was purchased from New England Biolabs (NEB). The Cas9 digestion reaction (30 μL) consisted of 1× Cas9 Nuclease Reaction Buffer, 30 nM Cas9 Nuclease (NEB), 30 nM sgRNA a (16–1274 or 18–1490; Table 1) and 30 nM sgRNA b (16–950 or 18–...
Exo Ⅰ and 10 × NEBuffer 3.1 (1 × NEBuffer 3.1, 100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 100 μg mL−1 BSA, pH 7.9) were purchased from New England Biolabs Ltd. (Beijing, China). Hydrogen tetrachloroaurate trihydrate (HAuCl4·3H2O), Tris (2-carboxyethyl) phosphine (TCEP),...
In short, a PCR fragment was amplified using the left and right primers sin_genot_F (5′-TCTTGAATGCTGCCTTTCTG-3′) and sin_genot_R (5′-GCCCTCAAGATATAAAAATGCAA-3′) and then digested using the restriction enzyme AjuI (New England Biolabs, Ipswich, MA, USA), which do not digest ...
For PCR amplification of DMRs, the Taq DNA polymerase (New England Biolabs) was used. Table S2: List of primers used for expression analyses of rice genes located nearby DMRs. Details on real-time RT-PCR conditions are described in material and methods. Author Contributions Conceptualization: L...