Eight years ago, 10x Genomics launched the first commercially available single-cell RNA sequencing (scRNA-seq) product for the company’s flagship instrument, the 10x Chromium. (This occurred one year after the launch of GemCode—the Chromium’s earlier iteration.) The instrument was a game chang...
In recent years, a number of droplet-based platforms for high-throughput scRNA sequencing have attracted attention, including 10X Chromium Genomics, inDrop and Drop-seq [46,47,93]. These technologies share similar strategies in generating droplets, isolating single cells through on-bead primers w...
Each sample was processed on an independent Chromium Single Cell A Chip (10X Genomics) and subsequently run on a thermocycler (Eppendorf). 3’ and 5’ gene expression libraries were sequenced using the NextSeq 500 150-cycle high-output flow cells. B- and T- cell VDJ libraries were sequenced...
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Cancer is a disease largely caused by genomic aberrations. Utilizing many rapidly emerging sequencing technologies, researchers have studied cancer genomes to understand the molecular statuses of cancer cells and to reveal their vulnerabilities, such as
A specially designed holder with electrical contacts was employed to keep the sensor chip inside the reaction chamber in a way that the zeolite film is in the focal point of the infrared beam of the DRIFTS mirror design. Simultaneous IS and DRIFTS measurements were carried out using the same ...