All samples were analysed by using a BD FACSCanto flow cytometer (BD Biosciences, San Jose, CA), with the results subsequently evaluated using FlowJo Software (Version X; TreeStar, Ashland, OR). Generation and induction of bone marrow-derived dendritic cells The induction of murine bone marrow...
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November 15 through Saturday, November 18 LA TEMPÊTE, by William Shakespeare, directed by Michel Lemieux, Victor Pilon, and Denise Guilbault, a 4D art production HEDDA GABLER Brooklyn Academy of Music BAM Harvey Theater 651 Fulton Street between Ashland Pl. &...
director Luc Schaedler and cameraman Filip Zumbrunn reveal a different side of the supposedly peaceful Shangri-La by focusing on a radical, subversive monk who had a completely different perspective of his homeland. Born in 1903 near the eastern border with China, Gendun Choephel...
Ashland Inc., Wilmington, DE, USA donated KlucelTM Hydroxypropyl cellulose (HPC) JF grades. Soluplus® was a gift from BASF, Ludwigshafen, Germany. All other chemicals and solvents used in the study were of analytical grade or high-performance liquid chromatography (HPLC) grades. 2.2. Preparati...
(1) Background: Lactoferrin has been recognized as a potent inhibitor of human herpetic viruses, such as herpes simplex type 1 (HSV-1) and 2 (HSV-2). In this work, we tested if silver and gold nanoparticles modified with lactoferrin (LF-Ag/AuNPs) can bec
into 35-mmglass-bottomdishes (MatTek, Ashland, MA) and were then treated with 400 lM PA for 48 h. After the treatment, the cells were washed once with PBS and then incubated with 200 ll 1Âbinding buffer containing 1 ll Annexin V-EGFP and 1 ll PI at room temperature for 5 min...
cells and were done with FlowJoVersion 7.6 (Ashland, OR, USA). 2.5. Detection of cytokine production with multiplex assay After 24-hour incubation, all LGG bacteria were engulfed by DCs. DCs were washed with PBS and incubated with autologous ...
(Ashland, MA, USA). UFP-512, a specific and potent DOR agonist (Balboni et al., 2002; Chao et al., 2007a) was synthesized by our research team. Cell culture Due to the endogenous expression of α-synuclein and the dopami- nergic characteristics of HEK293T cells, HEK293/α-...