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For qPCR, the SYBR Green PCR master mix (Thermo Fisher Scientific, 4367659) was used. And qPCR was carried out using the StepOnePlus real-time PCR system (Applied Biosystems). Gene expression was normalized to the endogenous control (β-actin). The following primers were used: β-actin (F...
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Cancer early detection and treatment response prediction continue to pose significant challenges. Cancer liquid biopsies focusing on detecting circulating tumor cells (CTCs) and DNA (ctDNA) have shown enormous potential due to their non-invasive nature and the implications in precision cancer management....
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