Positioning of bone marrow hematopoietic and stromal cells relative to blood flow in vivo: Serially reconstituting hematopoietic stem cells reside in distinct nonperfused niches Blood, 116 (2010), pp. 375-385 View PDFView articleCrossrefView in ScopusGoogle Scholar Wojcikiewicz et al., 2009 E.P...
Relative expression among different cells is shown. d, Graphic representation of immune cell proportions in KEP tumours (n = 4). e, Primary tumour growth kinetics of control and γδ T cell-depleted tumour transplant recipient mice (n = 13 per group). f, Growth kinetics of primary tumours ...
aStrong correlation of gene expression between resting human (x) and mouse (y) blood neutrophils. Left: all genes (r = 0.84,P < 2.2e-16). Middle: transcription factors, highlighted in green (r = 0.87,P < 2.2e-16). Transcription factors were retrieved from a curated ...
They were kept with a 12 h daily lighting, 50%-70% relative humidity, and at a temperature of 19–23°C in type-3 cages. Mice received autoclaved food (#1314, Altromin, Germany) and water (adjusted with HCl to pH 2.5–3.0 to prevent growth of microorganisms). As the genetic ...
The relative mRNA expression levels of all known kinds of mammalian VGCC were determined in human neutrophils by real-time RT-PCR. Total neutrophil RNA was extracted using RNAiso Plus (TaKaRa, Dalian, China) according to the manufacturer’s instructions. For real-time quantification, first strand...
These cells were then suspended in PBS and cell suspension were counted using haemocytometer and number of cells were adjusted to a final concentration of 1 × 106 cells/mL. The use of human blood was approved by the Human Ethical Committee, Faculty of Medicine, Universiti Kebangsaan Malaysia ...
The relative mRNA levels of the target genes were calculated by 2−ΔΔCT. Table 1 Primers used in the RT-qPCR Full size table ELISA analysis of cytokine expression PMNs were incubated with or without Ad3 ESP (10 ng/μl) for 3 h, and supernatants were harvested for cytokine analysis....
However, the plasticity of neutrophil phenotypes and their relative impact on COVID-19 pathogenesis has not been well addressed. This study aimed to identify and validate the heterogeneity of neutrophils in COVID-19 and evaluate the functions of each subpopulation. We analyzed public single-cell ...
For morpholino experiments where neutrophils were absent, the XY position of each fluorescent bacterial signal was identified based on thresholding and particle analysis, with distribution distance calculated relative to the largest fluorescent object, which represented the infection bolus. Statistics All ...
7.The method of claim 5, wherein the labeled count for each subpopulation from the plurality of subpopulations of neutrophils is:i) displayed proximate the labeled neutrophil count in the interface reporting results of the first test; andii) indented at a first level relative to the labeled neu...