The action of H2O2 on intact cells is therefore likely to be caused by generation of intracellular ADPR which then activates the TRPM2 ion channel (see diagram in Supplementary Materials Fig. 8). Leading-edge calcium pulses in H2O2 and ADPR are driven by TRPM2 The results above suggest ...
Furthermore, it has been previously established that the type of cell death of ingested host cells can dictate the inflammatory response of professional phagocytes on engulf- ment. Macrophage ingestion of apoptotic cells has been shown to be non-phlogistic, while necrotic and autophagic cells ...
Although we were unable to detect a significant increase in the total abundance of glutamine in response to LPS (Figure 1M), we found that LPS upregulated glutamine conversion into glutamate (Figure 1N), indicative of increased glutamine utilization in LPS-stimulated neutrophils. Download: Download ...
Periodontal ligament stem cells (PDLSCs) are promising cell resource for the cell-based therapy for periodontitis and regeneration of bio-root. In this study, we investigated the effect of PDLSCs on neutrophil, a critical constituent of innate immunity, and the underlying mechanisms. The effect of...
In healthy individuals, the immune system is able to detect and eliminate emerging and fully developed tumor cells. This protective system fails, if tumor cells gain characteristics by which they can evade the immune surveillance; they become invisible to the immune system and a clinically ...
PMNs employ phagocytosis, an essential function to detect, engulf, and destroy microbes, including bacteria, fungi, protozoa, and parasites, using their plasma membrane to form a phagosome that fuses with lysosome-containing enzymes and toxic substances to eliminate the microbe [2,3,4]. This ...
Whilst it was possible to select neutrophils from this population that exhibited intact plasma membranes, many of the cells showed one or more points of loss of integrity. Whether this represents the fragility of these cells during processing for electron microscopy is not clear. However, what is...
S100A8/S100A9 is a proinflammatory mediator released by myeloid cells during many acute and chronic inflammatory disorders. However, the precise mechanism of its release from the cytosolic compartment of neutrophils is unclear. Here, we show that E-selec
Because activated neutrophils can potently produce ROS and phagocyte NADPH oxidase 2 complex-derived ROS are known to be crucial regulators of immune response25,26, we asked whether ROS might mediate the regulation of the macrophage skewing. Using the fluorescent probe CM-H2DCFDA to detect intracellu...
such as, for example, the CD16 antibody. Using this method EGCs can be identified based upon the lack of CD16 staining on EGC cells. However, the method involves the use of multiple antibodies to sequentially gate different cell types. Therefore, the flow cytometric ...