we calculated the differences in the module’s expression levels (“eigengene differentials”) between healthy state and moderate influenza, or between moderate influenza and severe influenza (Fig.3c). This calculation revealed that, as infection severity increased from moderate to severe, the neutrophi...
Endogenous peroxidase activity was blocked for 15 min using absolute methanol containing 3% hydrogen peroxide. After washing the sections in PBS, they were microwaved for 10 min to achieve antigen retrieval. Non-specific binding was blocked using a non-specific staining blocking reagent (Dako, Kyoto...