There is a dearth of information defining hematopoietic development, including neutrophil leukocyte differentiation in this species using multicolor flow cytometry. In the current study, we sought to identify cell surface markers that delineate neutrophil progenitor populations with characteristic immunophenotype...
b, feature plots of marker genes commonly used in the literature to define BM progenitor cell subsets. c, bubble chart showing the expression of marker genes in each cell cluster. d, UMAP clustering of BM Lin- cells from non-exposed and HFD exposed mice (n = 4). Lin- BM cells ...
A source of neutrophils, the most abundant immune cells, has been found. Researchers at La Jolla Institute for Allergy and Immunology identified a progenitor of neutrophils in the bone marrow of mice and humans and tied it to cancer-promoting activities. The findings could assist in the detection...
et al. Identification of an early unipotent neutrophil progenitor with pro-tumoral activity in mouse and human bone marrow. Cell Rep. 24, 2329–2341.e8 (2018). CAS PubMed PubMed Central Google Scholar Fridlender, Z. G. et al. Polarization of tumor-associated neutrophil phenotype by TGF-...
c-Kit and CD34 were used to exclude CD34 – c-Kit+ stem/progenitor cells and FSC/SSC gating excluded eosinophils (SSChigh). The differential expression levels of c-kit and Ly6G were used to define the developmental stages of neutrophils. “Fluorescence minus one” (FMO) controls were ...
(mouse progenitor Lin−, CD117+, Ly6A/E+, with additional markers: Siglec F-, FcεRIα-, CD16/CD32+, Ly6B+, CD162lo, CD48lo, Ly6C-, CD115-; human: CD66b+, CD117+, CD38+ CD34+/−). Neutrophils go through distinct developmental stages...
(G-CSF) and an interaction of G-CSF/G-CSF-receptor is necessary for both the proliferative response of hematopoietic stem andprogenitor cells(HSPC) and the mobilization of bone marrow neutrophils (Cain et al., 2011; Dale et al., 2008).Scumpia et al. (2010), on the other hand, showed...
(mouse progenitor Lin−, CD117+, Ly6A/E+, with additional markers: Siglec F-, FcεRIα-, CD16/CD32+, Ly6B+, CD162lo, CD48lo, Ly6C-, CD115-; human: CD66b+, CD117+, CD38+ CD34+/−). Neutrophils go through distinct developmental stages...
(proNeu1) in mice. proNeu1 gives rise to proNeu2 progenitor, subsequently differentiating into a proliferative neutrophil precursor (preNeu), which finally develops into immature neutrophils and mature neutrophils. In homeostasis, CXCR4, a retention signal in the bone marrow, gradually downregulated ...
Here we report the identification of human CD66b−CD64dimCD115− neutrophil-committed progenitor cells (NCPs) within the SSCloCD45dimCD34+ and CD34dim/− subsets in the bone marrow. NCPs were either CD45RA+ or CD45RA−, and in vitro experiments s