The characteristics, culture conditions and usefulness of the human neuroblastoma cell line SH-SY5Y as a model for axonopathy are presented in this chapter. It is described how the cell line can be differentiated to mature neurons by using serum-free "N2" medium supplemented with retinoic acid....
Likewise, undifferentiated SH-SY5Y has also been chosen as a model cell line for studies in neuroscience. 5.44.2.2 Primary Neuronal Cells Primary cultured neuronal cells are mainly dissociated from peripheral or CNS tissue. These cells retain many of the morphological, neurochemical, and ...
method, the SH-SY5Y neuroblastoma cell line is differentiated to neuronal cell which expresses NSE, neuronal marker, and dopamine transporter (DAT) by treatment with all-trans-retinoic acid. SH-SY5Y cells allow investigating neuronal phenotypes of mental disorders as an in vitro model of these ...
To obtain some insight into the mechanisms of cell death involved in neurodegenerative diseases, we studied the effect of 1-methyl-4-phenylpyridinium (MPP+) on the human neuroblastoma cell line SH-SY5Y (a widely used model of Parkinson's disease). We found that MPP+ predominantly induced non-...
In the present study, we investigated ER stress and neuronal apoptosis following METH treatment and the role of melatonin in METH-mediated ER stress-induced cell death in the SH-SY5Y neuroblastoma cell line. We found that METH caused the overexpression of ER stress-related genes, including C/...
These cells were originally derived from the SK-N-SH cell line which was sub-cloned three times to SH-SY, SH-SY5 and finally to the SH-SY5Y cell line. SH-SY5Y cells are often used in their undifferentiated state and these cultures contain both floating and adherent cells. The adherent ...
C-peptide enhances insulin-mediated cell growth and protection against high glucose-induced apoptosis in SH-SY5Y cells. C-peptide in the presence of insulin exerts synergistic effects on cell proliferation, neurite outgrowth and has in the presence of insulin an antiapoptoti... ZG Li,W Zhang,AAF...
induced neuronal death may recruit an array of candidate mediators other than TRAIL and DR5.29,33,34 All together, these data provide evidence that the TRAIL/ DR5/FADD/caspase-8 pathway acts as an efficacious de- structive effector system in the human neuronal cell line SH- SY5Y exposed to ...
To obtain some insight into the mechanisms of cell death involved in neurodegenerative diseases, we studied the effect of 1-methyl-4-phenylpyridinium (MPP+) on the human neuroblastoma cell line SH-SY5Y (a widely used model of Parkinson's disease). We found that MPP+ predominantly induced non-...
In the present study, we investigated ER stress and neuronal apoptosis following METH treatment and the role of melatonin in METH-mediated ER stress-induced cell death in the SH-SY5Y neuroblastoma cell line. We found that METH caused the overexpression of ER stress-related genes, including C/...