Stem Cell Reports. 2019;12(3):518–531. Breast A CD146 FACS protocol enriches for luminal keratin 14/19 double positive human breast progenitors Ísberg et al. Sci Rep. 2019; 9:14843. Colon Modeling the effect of prolonged ethanol exposure on global gene expression and chromatin ...
Modeling G2019S-LRRK2 sporadic Parkinson's disease in 3D midbrain organoidsKim et al.Stem Cell Reports.2019;12(3):518–531. BreastA CD146 FACS protocol enriches for luminal keratin 14/19 double positive human breast progenitorsÍsberg et al.Sci Rep.2019; 9:14843. ...
Efficient neuronal differentiation of mouse ES and iPS cells using a rotary cell culture protocol. Differentiation, 86(4-5):149-58, 2014.Mohamad O, Yu SP, Chen D, Ogle M, Song M, Wei L (2014) Efficient neuronal differentiation of mouse ES and iPS cells using a rotary cell culture ...
Figure 1.Protocol for Plating and Culturing Primary Neurons with the SM1 Culture System Primary rodent tissue dissociated in papain was plated in NeuroCult™ Neuronal Plating Medium, supplemented with NeuroCult™ SM1 Neuronal Supplement, L-Glutamine, and L-Glutamic Acid. On day 5, primary neuron...
It establishes that uncharged copolymers lead to MGPs with nonfouling properties inappropriate for cell culture, and it provides a protocol to amend their surface properties to enable cell adhesion. Finally, it demonstrates that the introduction of positive charges by METAC is necessary to obtain ...
This protocol describes how to perform single-cell axotomy on dissociated hippocampal neurons containing synapses. Furthermore, to axotomize hippocampal neurons integrated in neuronal circuits, we describe how to set up coculture with a few fluorescently labeled neurons. This approach allows axotomy of...
These glial cells adhere to the collagen droplets and, over time in culture, come to occupy nearly the entire extent of the droplet. Neurons appear to adhere to the glial cell in small colonies of one or more cells. Importantly, the agarose substrate does not permit either cell adhesion ...
cell cultureneuronal migrationCultures of cerebellar granule cells are a suitable model to analyze the mechanisms governing neuronal migration. In this unit, we describe a protocol to obtain cultures of dissociated granule cells at a low density, where individual cells can be easily observed. In ...
Modeling G2019S-LRRK2 sporadic Parkinson's disease in 3D midbrain organoidsKim et al.Stem Cell Reports.2019;12(3):518–531. BreastA CD146 FACS protocol enriches for luminal keratin 14/19 double positive human breast progenitorsÍsberg et al.Sci Rep.2019; 9:14843. ...
Protocol D: V41 was diluted 1:3.5 in 50 µl cell-containing medium. Cells were expanded and maintained as described above. After two weeks, the obtained H4_41 cells were transduced with V99. For all protocols, cell nuclei were stained with 0.5 µg/ml Hoechst33342 (Invitrogen, H...