For each sample, 50 ng total RNA was used to prepare Cy3-labelled target cRNA with the Low Input Quick Amp Labeling Kit (Agilent Technologies). The target cRNAs were hybridized to SurePrint G3 Mouse GE 8×60K Microarrays (AMADID 028005, Agilent Technologies). Scanned images were analysed ...
2.12. Light-Sheet Imaging For light-sheet imaging, zebrafish embryos at the desired developmental stage were manually dechorionated and anesthetized in 0.04 mg/L tricaine and mounted in a glass capillary containing 1% low melting agarose (16520050, Thermo Fisher Scientific). After polymerization, capi...