A case–control study was conducted. Cancer cases were recruited from Children’s Healthcare of Atlanta and healthy controls were recruited via flyers. Participants reported PNS using the Pediatric Patient-Reported Outcomes Measurement Information System. Data for cases were collected pre-cycle two chemo...
To do this, the mRNA template is removed and a second, complementary strand of DNA is synthesized using a DNA-dependent DNA polymerase. Once double-stranded cDNA has been synthesized, the collection of molecules must be inserted into vectors so that they can be further manipulated. Vectors, ...
OGMS utilizes a template for generating unambiguous textual and formal logical definitions for terms in an effort to promote the integration of scientific data. ND seeks to produce definitions in a similar manner within the domain of neurological disease. We are developing the Neurological Disease ...
A case–control study was conducted. Cancer cases were recruited from Children’s Healthcare of Atlanta and healthy controls were recruited via flyers. Participants reported PNS using the Pediatric Patient-Reported Outcomes Measurement Information System. Data for cases were collected pre-cycle two chemo...
NATs, as the name suggests, are single-stranded RNAs transcribed from a DNA template strand in the opposite direction to that of its sense transcript [50]. NATs are typically 200–400 nucleotides in length and usually do not contain an open reading frame (ORF), although some exceptions have...
The genomic DNA from each individual was NATURE GENETICS VOLUME 36 | NUMBER 4 | APRIL 2004 367 ARTICLES used as a template for PCR to amplify each coding exon of the SOX10 gene1. We sequenced PCR products directly by using DyePrimer chemistry and an ABI377 sequencer (Applied Biosystems). ...
In August 2016, a new SRC-specific working group (WG) began developing and identifying CDEs, template case report forms (CRFs), and guidelines to assist investigators conducting SRC-specific clinical research studies. Design/methods The CDE WG, which consisted of 34 worldwide SRC research ...
HR uses a homologous copy of the sequence, for example, a sister chromatid, as a template to achieve precise, error-free repair. HR repair relies on extensive DNA processing at the break site via MRN complex and CtIP, in order to generate 3' single stranded DNA where Rad51 can assemble ...
This SOP is meant to be a comprehensive, yet not too detailed template to allow for freedom in local adaption. It comprises all relevant stages in providing care to the patient with severe ischemic stroke such as suspicion and alarm, prehospital acute measures, recognition and grading, transport...
Precise changes can be introduced into the genome using CRISPR/Cas9 if a homologous DNA repair template is supplied at the same time, permitting repair by the high-fidelity homology-directed repair (HDR) pathway (Cong et al. 2013; Paquet et al. 2016). Unfortunately, the therapeutic potential ...