If no template is yet available for this journal, please follow the format of the sample references and citations as shown in this Guide. If you use reference management software, please ensure that you remove all field codes before submitting the electronic manuscript. More information on how ...
To do this, the mRNA template is removed and a second, complementary strand of DNA is synthesized using a DNA-dependent DNA polymerase. Once double-stranded cDNA has been synthesized, the collection of molecules must be inserted into vectors so that they can be further manipulated. Vectors, ...
A case–control study was conducted. Cancer cases were recruited from Children’s Healthcare of Atlanta and healthy controls were recruited via flyers. Participants reported PNS using the Pediatric Patient-Reported Outcomes Measurement Information System. Data for cases were collected pre-cycle two chemo...
This SOP is meant to be a comprehensive, yet not too detailed template to allow for freedom in local adaption. It comprises all relevant stages in providing care to the patient with severe ischemic stroke such as suspicion and alarm, prehospital acute measures, recognition and grading, transport...
Precise changes can be introduced into the genome using CRISPR/Cas9 if a homologous DNA repair template is supplied at the same time, permitting repair by the high-fidelity homology-directed repair (HDR) pathway (Cong et al.2013; Paquet et al.2016). Unfortunately, the therapeutic potential of...
NATs, as the name suggests, are single-stranded RNAs transcribed from a DNA template strand in the opposite direction to that of its sense transcript [50]. NATs are typically 200–400 nucleotides in length and usually do not contain an open reading frame (ORF), although some exceptions have...
The genomic DNA from each individual was NATURE GENETICS VOLUME 36 | NUMBER 4 | APRIL 2004 367 ARTICLES used as a template for PCR to amplify each coding exon of the SOX10 gene1. We sequenced PCR products directly by using DyePrimer chemistry and an ABI377 sequencer (Applied Biosystems). ...
During DNA replication, the 8-hydroxyguanosine complements the adenosine-containing template. The rate of this mutation depends upon the proportion of oxidized DNA damage resulting in the base pair mismatch. The mismatched pairing leads to translation of abnormal proteins (Figure 4). This whole ...
Authors preparing their manuscript in LaTeX can use the bibliography style file sn-basic.bst which is included in theSpringer Nature Article Template. Back to top Tables All tables are to be numbered using Arabic numerals. Tables should always be cited in text in consecutive numerical order. ...
(Illumina, FC-131-1001). Clean-up was performed on the indexed libraries using AMPure XP beads. The 16S libraries were pooled in equal amounts based on fluorescence quantification. Each run included a control template to test for polymerase chain reaction (PCR) accuracy and possible ...